摘要
目的:为了得到高F值寡肽,需要对蛋白质进行水解。方法:采用酶法水解魔芋飞粉制备高F值寡肽。通过对蛋白质水解度的测定,确定了碱性蛋白酶和链霉蛋白酶的最佳酶解条件:碱性蛋白酶加酶量0.1%,底物浓度3.75%,pH9.0,水解温度45℃,时间5h;链霉蛋白酶加酶量0.03%,pH8.0,水解温度50℃,时间7h。酶解液再经过凝胶层析分离纯化后可用于制备高F值寡肽。
In order to get oligo - peptides of high fischer ratio, The protein should be hydrolyzed. In this paper ,we get oligo - peptides of high fis- cher ratio from konjac fly powder which was hydrolyzed with protease. The optimum conditions of alkaline protease and pronase were established from the degree of hydrolysis： the quantity of alkaline protease is 0.1%, the density of substratum is 3.75 %, pH is 9.0, the hydrolysis temperature is 4593 and the reactive time is 5 hours; the quantity of pronase is 0.03%, pH is 8.0, the hydrolysis temperatuer is 5093 and the reactive time is 7 hours. The hydrolysis fluid would get oligo - peptides of high fischer ratio from separated by gelatin.
出处
《生物技术》
CAS
CSCD
2006年第3期67-69,共3页
Biotechnology
基金
湖北省科技攻关项目("魔芋飞粉开菲尔发酵乳研究"
012B0097)
关键词
魔芋飞粉
高F值寡肽
酶解
konjac fly powder
oligo- peptides of high fischer ratio
enzymatic hydrolysis
作者简介
赵珊珊（1980-），女，江苏南京人，硕士研究生，专业方向：分子食药生物工程，E-mail：zss-19800912@163．com；
干信（1942～），男，湖北武穴人，教授，博士生导师，研究方向：分子食药生物工程，获省奖2项、专利3项，发表专著2部。
