摘要
目的:探讨花叶开唇兰的活性氧清除作用。方法:制备花叶开唇兰水提液(ARE)、石油醚提取部位(AP)、氯仿提取部位(AC)、乙醇提取部位(AE)和水提取部位(AW),分别用NBT光化还原法、Fenton反应法和钼酸铵显色分光光度法检测对O2·-、·OH及H2O2活性氧的清除作用。结果:ARE对O2·-、·OH具有清除作用,曲线为抛物线型,EC50分别为1.72mg/ml和1.94mg/ml;ARE对H2O2无明显清除作用。AW对O2·-和·OH的清除作用明显高于AP、AC和AE。结论:花叶开唇兰具有清除O2·-和·OH的能力,活性主要存在于其水提取部位中。
Object： To evaluate the ability on scavenging of reactive oxygen species by Anoectochilus roxburghii（Wall.）Lind. Methods： preparation aqueous extraction of Anoectochilus roxburghii（Wall.）Lindl. （ARE）, extractions by petroleum（AP）, chloroform（AC）, ethanol（KE） and water（AW） of Anoectoehilus roxburghii（Wall.） Lindl. The scavenging of O2^-、·OH and H2O2 was estimated separately by methods based on nitro blue terra-zolium photochemical reduction, Fenton reaction and spectrophotometric assay. Results： ARE had capacity of scavenging O2^- and·OH generated separately by system of NBT photochemical reduction and Fenton reaction. The EC50 for ARE was separately 1.72mg/ml and 1.94mg/ml. while the capacity of scavenging H2O2 was low. The capacity of scavenging O2^- and·OH by AW was obviously higher than by AP, AC and AE. Conclusion： The extraction from Anoectochilus roxburghii（Wall.） Lindl. had ability of scavenging O2^- and·OH and the ability has been assigned to AW mainly.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第2期39-41,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
贵州省高校发展专项资金自然科学类重点项目(黔教科2004110)
关键词
花叶开唇兰
活性氧
Anoectochilus roxburghii（Wall.）Lindl.
scavenging
reactive oxygen species
