摘要
目的探讨原发性胆汁性肝硬化(PBC)患者出现的线粒体抗体(AMA)亚型与PBC患者肝功能损伤的相关性。方法用间接免疫荧光法检测52例PBC患者的AMA抗体,用酶联免疫吸附试验(ELISA)检测AMA的M2,M4和M9抗体。结果52例PBC患者AMA和M2抗体均为阳性,M4和M9抗体阳性例数分别是40例(76.9%)和11例(21.2%)。M4阳性的PBC患者的ALT、AST和IgM的水平明显高于M4阴性患者。M9阳性的PBC患者的ALT、AST和IgG的水平明显低于M9阴性患者。结论AMA-M2亚型抗体检测对PBC有诊断价值,M4和M9抗体的检测,对于PBC的病情判断有意义。
Objective To study the significance of anti-mitochondria antibedy＇ s subtypes anti-M2,anti-M4,and anti-M9 in diagnosing primary biliary cirrhosis.Methods Anti-mitochondrial antibody was detected by indirect immunofluorescence and anti-M2,anti-M4 and anti-M9 by EIASA. Results 100% （52/52） of PBC patients were AMA and anti-M2 positive, 76.9% （40/52） of PBC patients were anti-M4 positive and 21.2% （11/52） of PBC patients were anti-M9 positive.The level of ALT, AST and IgM in patients with anti-M4 are higher than that in patients without anti-M4. Meanwhile the level of ALT, AST and IgG in patients with anti-M9 are lower than that in patients without anti-Mg. Conclusion AMA-M2 detection has definitive value to diagnosis PBC. AMA-M4,M9 detection show significance to analyze the stage of PBC.
出处
《中国实验诊断学》
2006年第6期621-623,共3页
Chinese Journal of Laboratory Diagnosis
基金
基金资助:北京市科委科技计划项目:(H020920020190)
作者简介
通讯作者：闫惠平，首都医科大学博士研究生导师．
