摘要
目的探讨就诊人群中抗核抗体(ANA)和核型的分布趋势,及其在疾病中的分布与诊断的意义。方法用间接免疫荧光法(IIF)检测1 000例标本中的ANA,结合临床资料分析ANA在不同年龄、性别和疾病中的分布趋势和各种核型在不同疾病的分布趋势及临床意义。结果患者检出ANA的阳性率的46.5%且女性高于男性,50岁以上患者高于50岁以下患者。ANA阳性率在系统性硬皮病(SSc)占100%,系统性红斑狼疮(SLE)93.3%,原发性胆汁性肝硬化(PBC)85.7%,干燥综合征(SS)78.1%,类风湿关节炎(RA)61%,强直性脊柱炎(AS)15%。结论 ANA在SSc、SLE、PBC、SS、RA中阳性率较高,核型和抗体滴度对诊断和监测疗效有临床意义。因核型可重叠交叉表现,应重视抗体滴度的诊疗作用,避免片面根据某种核型弱阳性所致的误诊。
Objective To explore the positive distribution of anti-nuclear antibodies（ANA）and various nuclear-types in population and different diseases,and their significance for clinical diagnosis.Methods Indirect immunofluorescence assay（IIF） was performed for the detection of ANA in 1 000 cases of clinical specimens.The positive distribution of ANA in population of different ages,sexes and with different diseases,and the distribution of nuclear-types in different diseases were investigated.Results The positive rate of ANA in patients was 46.5%.The positive rate of ANA was significantly higher in female than in male（P0.01）and higher in patients more than 50 years old than in patients less than 50 years old（P0.01）.The positive rate of ANA was 100% in systemic scleroderma（SSc）,93.3% in systemic lupus erythematosus（SLE）,85.7% in primary biliary cirrhosis（PBC）,78.1% in Sjogren syndrome（SS）,61% in rheumatoid arthritis（RA） and 15% in ankylosing spondylitis（AS）.Conclusion The positive rate of ANA in SSc,SLE,PBC,SS and RA could be high,and analysis of nuclear-types and the titer of antibodies could have certain clinical significance for diagnosis and evaluation of therapeutic effects.More attention should be paid for analysis of the titer of antibody to avoid misdiagnosis,caused by weakly positive nuclear-type,for there might be overlap of different nuclear-types.
出处
《国际检验医学杂志》
CAS
2011年第18期2072-2073,2076,共3页
International Journal of Laboratory Medicine
关键词
自身抗体
抗体
抗核
自身免疫疾病
autoantibodies
antibodies
anti-nuclear
autoimmune diseases
