摘要
背景与目的国外研究表明不论以何种方式用烟草特有亚硝胺4-(甲基亚硝胺)-1-(3-吡啶)-1-丁酮[4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone,NNK]处理小鼠均能特异地诱发小鼠肺癌,且多为肺腺癌,NNK是公认的吸烟者患肺癌的一个病因。本研究的目的是通过测定吸烟者尿中NNK的代谢产物4-(甲基亚硝胺)-1-(3-吡啶)-1-丁醇[4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol,NNAL]及其解毒产物4-(甲基亚硝胺)-1-(3-吡啶-N-氧化物)-1-丁醇[4-(methylnitrosamino)-1-(3-pyridyl-N-oxide)-1-butanol,NNAL-Gluc]的含量及NNAL-Gluc/NNAL比值来了解NNK特异的致肺癌性。方法用高压液相色谱仪(HPLC)和气相色谱-双极质谱联用仪(GC-MS/MS)对10例吸烟肺癌患者、8例健康吸烟者和4例健康不吸烟者的24h尿样进行分析。结果不吸烟者的尿中未测到NNAL和NNAL-Gluc,健康吸烟者尿中平均NNAL-Gluc/NNAL比值为4.95,吸烟肺癌患者为0.5。结论建立了在吸烟者尿中测定NNK代谢产物NNAL及NNAL-Gluc的方法。初步研究显示健康吸烟者对NNK的解毒能力明显大于吸烟肺癌患者,为在吸烟人群中筛选肺癌高危人群提供了检测手段。
Background and objective It was reported that tobacco-specific nitrosamine 4-（methylnitro- samino）-1-（3-pyridyl）-1-butanone （NNK） was a powerful pulmonary carcinogen, predominantly inducing adenocarcinoma of the lung in mouse. The aim of this study is to assay metabolites of NNK, which are 4-（methylnitrosamino）-1-（3-pyridyl）-1-butanol （NNAL） and its O-glucuronide （NNAL-Gluc）, and their ratio （NNAL-Gluc/NNAL） in smokers and non-smokers＇ urine, and to explore the carcinogenicity of NNK among different people. Methods Using high pressure liquid chromatograph （HPLC） and gas chromatograph-mass tadom （GC-MS/MS）, NNAL-Gluc and NNAL in 24 h urine were detected in 8 healthy smokers, 10 lung cancer smokers and 4 healthy non-smokers. Results Both of the two metabolites were not found in non-smokers＇ urine. The ratios of urine NNAL-Gluc/NNAL were greatly different among different smokers. The mean ratio of NNAL-Gluc/NNAL in healthy smokers was 4.95, and 0. 5 in lung cancer smokers. Conclusion The results provide the first evidence for metabolite detection of tobacco-specific nitrosamine in Chinese smokers＇ urine. The result suggests that detoxification ability of healthy smokers is higher than that of lung cancer smokers. It may provide a detective way to screen high risk people for lung cancer in smokers.
出处
《中国肺癌杂志》
CAS
2006年第3期231-235,共5页
Chinese Journal of Lung Cancer
基金
北京市科委高技术实验室(No.H010910180113)资助~~
关键词
烟草特有亚硝胺
肺肿瘤
代谢产物
Tobacco-specific nitrosamine Lung neoplasms Metabolite
作者简介
通讯作者：赖百塘，E-mail：baitanglai@yahoo．com．cn
