摘要
目的:研究完全睡眠剥夺对大鼠海马和皮质IL-1βmRNA表达的影响。方法:将大鼠放入1转／分钟转笼中,制作经历不同持续时间的完全睡眠剥夺(TSD)模型。30只大鼠随机分为5小组:对照组(正常对照CC组,环境对照 TC组)和睡眠剥夺组[TSD6h组,TSD1d组,TSD3d组(n=6只)]。采用RT-PCR方法检测大鼠海马和皮质IL- 1βmRNA表达。结果:大鼠海马区,TSD6h组和TSD1d组IL-1βmRNA的表达明显高于对照组,TSD3d组更为明显;皮质区TSD3d组表达也明显增多。结论:完全睡眠剥夺大鼠海马和皮质IL-1βmRNA表达增高,且随时间的延长而逐趋明显。睡眠短期剥夺IL-1βmRNA表达增高可能对脑细胞具有应激性保护作用,而长期剥夺后IL-1β持续增高可能对神经元有损伤作用。
Objective： To investigate the effects of total sleep deprivation on IL-1βmRNA expression in rat＇s hippocampus and cortex. Methods： Put the rats in the rotating drums whose rotating rate were 1/min to make the TSD model in different time course. Divided the 30 rats into total five groups randomly： antitheses group（CC group for normal and TC group for environment） and sleep deprivation group （ TSD6h, TSD1 d, TSD3d （n=6））. we adopt the approach of RT-PCR to test IL-1βmRNA expression in rat＇s hippocampus and cortex. Results： The IL-1βmRNA expression of TSD6h group and TSD1d group was significantly higher than antitheses group within the hippocampus , especially TSD3d group; and also the expression of TSD3d group was significant increased within the cortex. Conclusion. Total sleep deprivation on IL- 1βmRNA expression in rat＇s hippocampus and cortex was higher, and the longer the time course, the more significant the result was. The higher IL-1βmRNA expression maybe help to protect the brain cell after short-time sleep deprivation, but they maybe harm the nerve cell after overtime in deprivation.
出处
《脑与神经疾病杂志》
2006年第3期193-195,共3页
Journal of Brain and Nervous Diseases
作者简介
通讯作者：赵忠新教授
