摘要
目的 建立高效液相色谱法测定舒肝丸中延胡索乙素含量的方法.方法 色谱检测条件为色谱柱： Spherisorb C18（4.6 mm×250 mm,5 μm）;流动相：甲醇-水（75：25,0.05 mol·L^-1 磷酸氢二钠溶液调pH值为8,经孔径0.45 μm微孔滤膜过滤）;检测波长： 280 nm;柱温： 25℃;流速： 0.9 mL·min^-1.结果 延胡索乙素在1.76～21.12 mg·L^-1 范围内与相应的峰面积呈良好线性关系（ r＝0.999 8） .加样回收率98.6%,RSD为1.38%.结论 该方法操作方便、灵敏、稳定,与组方中其他药材成分无干扰,可作为该产品含量测定方法之一.
Objective To set up a new method for the determination of the content of tetrahydropalmatine in shugan pills with HPLC. Methods The chromatographic conditions： A Spherisorb C is （4.6 mm×250 mm, 5μm） served as the chromatographic column; methanol-water （75 ： 25, the pH value adjusted to 8 with 0.05mol·L^-1 Na2HPO4 solution） was the mobile phase, the mixture was filtered through a Millipore membrane filter with an average pore diameter of 0.45 p,m; UV detection wavelength, 280 nm; column temperature, 25℃ ; flow rate, 0. 9mL·min^-1. Results There was a good linear relationship between the corresponding peak areas and tetrahydropalmatine when its concentrations were in the range of 1.76 - 21.12 mg·L^-1 （ r = 0. 999 8 ）. The average rate of recovery was 98.6% with an RSD of 1.38%. Conclusion The method was shown to be handy, accurate and reproducible and did not interfere with other medicinal materials in the prescription. It may thus be used as one of the methods for the content determination of tetrahydropalmitine in shugan pills.
出处
《医药导报》
CAS
2006年第6期570-572,共3页
Herald of Medicine
基金
浙江省自然科学基金资助项目(编号:301-468)
作者简介
朱晓军（1961-）,女,浙江金华人,从事临床医药研究.电话：0579-3126276,E-mail：zxj0801@sina.com.
