摘要
以平菇PL-39为出发菌株,采用紫外线连续诱变、分离提纯、驯化等方法,以斜面菌丝生长速度和液体摇瓶菌丝生物量及发酵多糖总量为测定目标,筛选出了一株遗传上相对稳定的适应液体培养的平菇菌株———PL-3903。该菌株在综合PDA培养基上生长快,长势旺,菌丝体浓密纯白,经驯化后在液体培养基中发酵生活力强,生物量达2.49 g/100 ml,发酵多糖总量达3.38 g/100 ml。
In this experiment, Pleurotus ostreatus strain PL-39 was used as original strain and a new strain PL-3903 suitable for liquid culture was selected by means of UV continuously induced mutation, iselation, purification and dome stication, and through the measure of growth rate of slanthyhae and polysaccharides quantity of fermentation. The result showed that PL-3903 had a relatively genetic stability and can grow fast and well in the medium with enriched PDA. Its mycelia were dense and white, and its vitality in liquid medium was strong. The biomass was up to 2.49 g/100 ml and total polysaccharides quantity was up to 3.38 g/100 ml.
出处
《安徽农业科学》
CAS
北大核心
2006年第9期1858-1859,共2页
Journal of Anhui Agricultural Sciences
关键词
液体发酵
平菇
诱变选育
生物量
Liquid fermentation
Pleumtus ostreatus
Selection of induced mutation
Biomass
作者简介
王淑芳（1950-），女，山东平度人，教授，从事微生物学和食用菌方面的教学与科研工作。
