摘要
以从健康牙鲆肠道中分离筛选的乳杆菌L15(Lactobacillussp.L15)和嗜酸乳杆菌ATCC4356为实验材料,应用5mol/L LiCl提取其表面蛋白,利用蛋白印迹法鉴定出在L15表面蛋白中分子量为61.8kDa和54.6kDa的蛋白质分别参与对牙鲆和鲤鱼粘液的粘附过程,为新发现的粘附蛋白种类,将其命名为MAPPpo1和MAPPcc。ATCC4356中分子量分别为43.0kDa和63.3kDa的两个表面蛋白参与对牙鲆粘液的粘附,而分子量为43.0kDa的蛋白参与对鲤鱼粘液的粘附。同时,蛋白质印迹法显示,L15和ATCC4356在牙鲆和鲤鱼肠粘液中均具有相同的粘附受体,在牙鲆肠粘液中是分子量为29.7kDa和30.3kDa的两种蛋白质,而在鲤鱼肠粘液中只有分子量为26.2kDa的蛋白作为受体参与L15和ATCC4356的粘附过程。结果显示,乳杆菌对肠粘液的粘附不但具有菌种的特异性,而且也有宿主的特异性。
Lactobacillus L15, a strain of Lactobacillus sp. isolated from the intestinal tract of healthy Paralichthys olivaceus, and Lactobacillus acidlophilus ATCC4356 were used to investigate their specific adhesions. It was shown that cell surface proteins of two strains could be extracted by 5mol/L lithium chloride（ LiCI）. Two surface proteins with molecular weights of 61 .SkDa and 54.6kDa were identified to mediate the adhesion to intestinal mucus of Paralichthys olivaceus and Common carp respectively by the method of Western blotting, and the two novel proteins were named as MAPPpol and MAPPcc. In Lactobacillus acidlophilus ATCC4356,two proteins of 43.0kDa and 63.3kDa were identified to mediate the adhesion to intestinal mucus of Paralichthys olivaceus. Nevertheless, only 43.0kDa protein was identified to mediate the adhesion to intestinal mucus of Common carp. Furthermore, the mucus proteins which participated the adhesion as the bacteria receptors were identified by the same method and it was found that LI5 and ATCCA356 had the same receptors.There were 29.7kDa and 30.3kDa proteins in the intestinal mucus of Paralichthys olivaceus and only the 26.2kDa protein was testified in the intestinal mucus of Common carp. It is demonstrated that the adhesion of Lactobacillus to mucus is dependent on the strain specificity, as well as host specificity.
出处
《微生物学报》
CAS
CSCD
北大核心
2006年第3期397-400,共4页
Acta Microbiologica Sinica
关键词
乳杆菌
表面蛋白
粘附
粘液
蛋白印迹法
Lactobacillus
surface protein
adhesion
mucus
Western blot
作者简介
陈营（1964-），男，辽宁锦州人，在站博士后，主要从事动物微生态学和乳酸菌分子生物学研究，E-mail：cheny728@yahoo．com．cn； 两位作者对本文有同等贡献,
王蕾（1979-），女，山东潍坊人，硕士研究生，主要从事分子微生物学和细胞免疫学研究，E-mail：panda-95@163．com.
通讯作者.Tel：86-531-86180143；Fax：86-531-86180107；E-mail：anlg@sdnu．edu．cn
