摘要
目的研究三七总皂苷(total sapon ins of Panax notoginseng,PNS)对异丙肾上腺素所致大鼠心肌肥厚的保护作用。方法用异丙肾上腺素(isoproterenol,ISO)5 mg.kg-1.d-1,sc,连续7 d,建立大鼠心肌肥厚模型。造模第2天开始给大鼠腹腔注射PNS25和50 mg.kg-1.d-1,连续14 d,测定全心重量指数(HW/BW),左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量和一氧化氮合酶(NDS)活力;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果ISO模型组大鼠的HW/BW,LVI明显升高;左心室Hyp、AngⅡ含量增高;NO水平下降,诱生型NOS(iNOS)活力显著升高,结构型NOS(cNOS)活力显著降低。PNS能明显提高心肌组织NO水平和cNOS活力,降低胶原的含量及iNOS活力,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚。结论PNS对ISO所致大鼠心肌肥厚具有保护作用。
Objective To investigate the protective effects of total saponins of Panax notoginseng （PNS） on myocardial hypertrophy induced by isoproterenol （ISO） in rats. Methods Myocardial hypertrophy model of rats were induced by injection of ISO （5 mg·kg^-1·d^-1 ,sc） for 7d, from day 2, the rats were administered with PNS 25 and 50 mg·kg^-1·d^-1, ip for 14d,the control and ISO model groups were received saline injection. Then, the heart - weight （HW）, left ventricular weight （ LVW）, the ratio of HW/BW and LVW/BW（LVI） were measured; the hydroxyproline content of left ventricle, the level of nitric oxide（NO） and angiotensin（AngⅡ） in left ventricle were determined by spectrophotemetry and radioimmunoassay, respectively. Results In ISO model group ,HW/BW, LVI and the content of hydroxyproline and AngⅡ in left ventricle markedly increased; the NO level in the left ventricle decreased. Treatment with PNS significantly reduced the HW/BW, LVI, decreased the AngII and the hydroxyproline content, increased the level of NO. Conclusion PNS was shown to prevent remodeling of myocardial hypertrophy induced by ISO
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第4期506-507,共2页
Lishizhen Medicine and Materia Medica Research
基金
赣南医学院资助项目(No.200406)
关键词
三七总皂苷
异丙肾上腺素
心肌肥厚
保护作用
Total saponins of Panax notoginseng
Isoproterenol
Myocardial hypertrophy
Protective effect
作者简介
周青（1963-），女（汉族），江西赣州人，现任赣南医学院机能实验室实验师，主要从事心脑血管病理与中药药理工作．
