摘要
目的研究三七总皂苷(PNS)对异丙肾上腺素(isoproterenol,ISO)所致大鼠心肌肥厚的保护作用。方法5mg/kg.d连续7d建立大鼠心肌肥厚模型。造模第二天开始ipPNS25和50mg/kg.d连续14d,测定全心重量指数(HW/BW)、左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量,及Na+,K+-ATP酶和Ca2+-ATP酶活性;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果ISO模型组大鼠的HW/BW、LVI明显升高;左心室Hyp、AngⅡ含量增高,NO水平下降,Na+,K+-ATP酶和Ca2+-ATP酶活性降低。PNS能明显提高心肌组织NO水平及Na+,K+-ATP酶和Ca2+-ATP酶活性,降低胶原的含量,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚。结论PNS对ISO所致大鼠心肌肥厚具有保护作用。
Objective： To investigate the protective effects of total saponins of panax notoginseng （PNS） on myocardial hypertrophy induced by isoproterenol （ISO） in rats. Methods： Myocardial hypertrophy model of rats were induced by injection of ISO 5mg/kg·d sc for 7d. From day 2, the rat were administered with PNS 25 and 50mg/kg·d ip for 14d, the control and ISO model group were received saline injection. Then, the heart-weight （HW）, left ventricular weight （LVW）, the ratio of HW/BW and LVW/BW （LVI） were measured; the content of hydroxyproline and angiotensin （Angll） , the level of nitric oxide（NO） and the activity of Na^＋, K^＋ -ATPase and Ca^2＋ -ATPase in left ventricle were determined by spectrophotometry and radioimmunoassay respectively. Results： In ISO model group, HW/BW, LVI and the content of hydroxyproline and AngII in left ventricle markedly increased; the level of NO and the activity of Na^＋, K^＋ -ATPase, Ca^2＋ -AT- Pase in the left ventricle were decreased. Treatment with PNS significantly reduced the HW/BW, LVI, decreased the AngII and the hy- droxyproline content, increased the level of NO and the activity of Na^＋, K^＋ -ATPase and Ca^2＋ -ATPase. Conclusion： PNS was shown to prevent remodeling of myocardial hypertrophy induced by ISO in rats.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2005年第4期27-29,共3页
Pharmacology and Clinics of Chinese Materia Medica
