摘要
目的:观察针刺对抑郁症模型大鼠行为学和前额皮质单胺类神经递质的影响,分析针灸治疗抑郁症可能的作用机制。方法:实验于2003-10/2004-05在南京中医药大学动物实验中心、江苏省针药结合实验室完成。选择Open-Field法评分相近的40只成年SD雄性大鼠随机分为正常组、模型组、氟西汀组、针刺组,每组各10只。应用分养和长期不可预见性中等强度应激造成抑郁症模型。实验第21天,进入治疗阶段。正常组,每笼4只,常规饲养。模型组,孤笼,常规饲养。氟西汀组,按照1.8mg/kg给予氟西汀灌胃,1次/d,连续21d。针刺组,应用32号1.0寸针灸针,百会穴向前斜刺,神庭穴向上斜刺,进针深度约为2mm,内关直刺1mm,三阴交直刺5mm,每穴均匀捻转刺激30s,留针10min,1次/d,连续21d。以Open-Field法对照研究针刺对慢性应激诱导的抑郁症模型大鼠行为学的改变;应用高效液相电化学检测系统对照研究针刺对模型大鼠前额皮质单胺类神经递质的改变。结果:纳入大鼠40只、全部进入结果分析,无脱失。①造模后,模型组和各治疗组大鼠行为学评分显著低于正常组(P<0.01)。②治疗第7天针刺组行为学已接近正常组(P>0.05);治疗21d后,氟西汀组行为学较模型组明显改善(P<0.01),与正常组接近(P>0.05)。③治疗21d后,针刺组大鼠前额皮质内5-羟色胺和去甲肾上腺素含量较模型组明显升高[(18.97±1.52),(13.35±0.69)ng/g;(715.97±23.43)(587.40±23.2)ng/g;P<0.01],去甲肾上腺素含量与正常组接近[(790.38±22.05)ng/g,P>0.05],5-羟色胺尚低于正常组[(23.56±0.82)ng/g,P<0.05];氟西汀组5-羟色胺含量较模型组显著升高[(27.12±1.46)ng/g,P<0.01],去甲肾上腺素含量较模型组变化不大[(535.26±19.70)ng/g,P>0.05]。结论:针刺能够改善抑郁症模型大鼠的行为学异常,起效快,3周疗效与氟西汀相当;针刺改善抑郁症模型大鼠前额皮质单胺类神经递质系统功能的作用机制和规律与氟西汀治疗存在差异。
AIM： To observe effects of acupuncture on praxiology and monoamine neurotransmitter in frontal cortex of depressed model rats and analyze the mechanism of acupuncture on treating depression.
METHODS： The experiment was completed at the Animal Experimental Center of Nanjiug University of Traditional Chinese Medicine and Jiangsu Laboratory of Integrated Acupuncture and Medicine from October 2003 to May 2005. Totally 40 adult male SD rats were scored with Open-Field method and divided randomly into normal group, model group, flhoxetine group and acupuncture group with 10 in each group. Depressed models were established with separation, long-term unpredictability and moderate stress. Therapy was started on the 21^th day. Rats in normal group were fed with routine feed with 4 in each cage. Rats in model group were fed routinely with 1 in each cage. Rats in fluoxetine group were perfused with 1,8 mg/kg fluoxetine once a day for 21 days. Rats in acupuncture group were acupunctured with 32-sized 1.0 cun needle at Baihui sloping forwardly and Shenting sloping upwardly in depth of 2 mm, Neiguang in depth of 1mm, and Sanyinjiao in depth of 5 mm. Each acupoint was twirled for 30 s and needle was maintained for 10 minutes. The operation was performed once a day for 21 days. Open-Field method was used to observe the effect of acupuncture on behavior changes of depressed model rats induced by chroriic stress, and effect of acupuncture on changes of monoamine neurotransmitter in frontal cortex was detected with high performance liquid chromatography （HPLC-ECD）.
RESULTS： All 40 rats entered the final analysis without any loss. ① Praxiological scores of rats were lower in model group and therapeutic groups than those in normal group after modeling （P 〈 0.01）. ②Praxiology of rats in acupuncture group was similar to that in normal group on the 7^th day of treatment （P 〉 0,05）, and 21 days after treatment, praxiology in fluoxetine group was improved as compared with that in model group （P 〈 0.01） and similar to that in normal group （P 〉 0.05）. ③ 21 days after treatment, contents of 5-serotonin and levarterenol in frontal cortex were higher in acupuncture group than those in model group [（18.97±1.52）, （13.35±0.69） ng/g; （715.97±23.43）, （587.40±23.2） ng/g; P 〈 0.01]; content of levarterenol was similar to that in normal group [（790.38±22,05） ng/g, P 〉 0.05]; content of 5-serotonin was lower than that in normal group [（23.56±0.82） ng/g, P 〈 0.05]; content of 5-serotonin in fluoxetlne group was higher than that in model group [（27,12±1.46） ng/g, P 〈 0.01], and content of levarterenol was not changed as compared with that in model group [（535.26±19.70） ng/g, P 〉 0.05].
CONCLUSION： Acupuncture can improve the behavior abnormality of depressed model rats and the curative effects are comparable to fluoxetine 3 weeks later, There are different mechanisms and regularities on improving function of monoamine neurotransmitter between acupuncture and flouxetine.
出处
《中国临床康复》
CSCD
北大核心
2006年第15期129-131,共3页
Chinese Journal of Clinical Rehabilitation
基金
江苏省教育厅自然科学研究计划(03KJA360093)~~
作者简介
张建斌，男，1967年生，江苏省江阴市人，汉族，南京中医药大学第二临床医学院在读博士，主治医师，讲师，主要从事针灸治疗脑病的基础与临床方面的研究。
