摘要
[目的]探讨威灵仙注射液对骨关节炎模型动物关节软骨的组织学、胶原表型和超微结构的作用。[方法]建立骨关节炎动物模型,动物随机分为3组,S组、D组和K组,S组和D组分别注射威灵仙注射液和生理盐水,K组空白。分批处死动物取关节软骨标本,常规石蜡切片,HE/SOFC双染色,M ank in评分比较各组关节软骨损伤程度。免疫组化法(IHC)检测各组关节软骨基质Ⅰ、Ⅱ型胶原的表达情况。透射电镜(TEM)观察各组关节软骨超微结构变化。[结果]S组在各时段的评分均优于D组和K组;S组在各时点Ⅰ型胶原阳染面积低于D组和K组,Ⅱ型胶原阳染面积高于D组和K组;S组软骨在各时间点损伤程度都低于D组和K组,K组和D组损伤程度基本一致。[结论]威灵仙注射液可维持和促进软骨合成蛋白多糖与Ⅱ型胶原,对关节软骨具有保护作用。
[ Objective] To study the effect of sixpetal clematis root injection （WLX） on histology, collagen phenotype and ultramicrostructurc in cartilage of osteoarthritis animal model. [ Method] OA model animals were randomly divided into 3 groups： group S, group D and group K. Animals in group S received WLX and animals in group D received sterilized normal saline intramuscularly, group K was control and received nothing. After the animals were killed, cartilage specimen were obtained and sections were made and stained with HE/SOFG for Mankin score; the expression level of collagen Ⅰ and Ⅱ in matrix was tested by immunohistochemistry; change of uhramicrostructure of cartilage was observed with transmission electron microscope. [Result] Mankin score in group S was better than that in group D and K at each test period; result of IHC shows that positive stain area of collagen Ⅰ was lower in group S than that in group D and K, and positive stain area of collagen Ⅱ was higher than that in group D and K at each test period ; cartilage impairment degree in group S was lower than in group D and K, and impairment manifestation in group D and K was similar on the whole. [ Conclusion] sixpetal clematis root injection may protect articular cartilage by maintaining and promoting the chondrocyte synthesizing proteoglycan and type II collagen.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第5期360-363,i0001,共5页
Orthopedic Journal of China
作者简介
陈飞雁（1973-），男，上海人，主治医师，骨科学博士。研究方向：关节外科、脊柱外科。电话：（021）6248999x1201
