摘要
目的建立D-101型大孔吸附树脂的预处理方法。方法采用正交试验设计以洗脱液中甲苯、萘的量及其紫外吸收值为指标(254 nm),考察了D-101型大孔吸附树脂预处理过程中的影响因素。结果溶剂和温度是树脂预处理中两个关键因素。建立了D-101型大孔吸附树脂预处理的优选工艺为柱温为60℃,先用2%N aOH浸泡洗脱,再用乙醇浸泡洗脱,洗脱体积流量为2 BV/h,乙醇用量为3.5倍柱体积(BV),此时乙醇洗脱溶液的吸光度约为0.4。结论该D-101型大孔吸附树脂的预处理工艺简单、生产周期短、环境污染小且预处理过程可用UV在线监测。
Objective To develop a method for the pretreatment of D-101 macroporous adsorption resin （MAR）. Methods The influence factors of pretreatment conditions were studied by orthogonal test according to the content of toluene and naphthalene in the eluate and its UV ahsorhance （254 nm）. Results Solvent and temperature were two key factors to the pretreatment process. The optimized method was established. D-101 MAR was eluted by 2% NaOH for 2 bed volumes （BV） of MAR and then by ethanol for only 3.5 BV at 2 BV/h at 60℃. At the end of elution, the UV absorbance of ethanol eluate was about 0.4. Conclusion The operation of D-101 MAR pretreatment is easy and the cycle is short. The process has little contamination to the environment and can be monitored by a on-line UV monitor.
出处
《中草药》
CAS
CSCD
北大核心
2006年第2期193-196,共4页
Chinese Traditional and Herbal Drugs
基金
"十五"国家科技攻关项目(2001BA701A02)
关键词
D-101型大孔吸附树脂
预处理
甲苯
萘
D-101 macroporous adsorption resin （D-101 MAR）
pretreatment, toluene
naphthalene
作者简介
夏存勤（1973-），男，甘肃农业大学2003届硕士，现北京大学药学院2003级在读博士生。E—mail：jiacunqin@tom．com
通讯作者 屠鹏飞Tel：（010）82802750 E—mail：pengfeitu@bjmu．edu．cn
