摘要
目的探讨门静脉部分动脉化对部分肝切除大鼠肝脏的影响。方法将4 8只SD大鼠分为肝部分切除术后非门静脉动脉化组及门静脉动脉化组。动态观察术后2,6,1 2 h血清ALT,AST,以及肝组织中ATP,ADP,AMP含量的变化,并计算EC值;同时取肝组织行病理组织学检查。结果(1)与非动脉化组比较,动脉化组血清AST和ALT在术后2 h无差异(P>0.0 5),术后1 2 h动脉化组血清AST和ALT动脉化组明显降低(分别为P<0.0 1及P<0.0 5)。(2)动脉化组较非动脉化组术后各时点肝组织ATP和EC均有明显增加(分别为P<0.0 1及P<0.0 5)。(3)非动脉化组肝组织病理变化随着缺血时间延长而加重;动脉化组病理变化较轻。结论门静脉部分动脉化在一定程度上可以减轻大鼠部分肝切除并肝动脉离断后的肝损害,改善肝细胞的能量代谢。
Objective To study the effect of partial portal vein arterialization on the liver of rats after partial hepatectomy. Methods Forty eight SD rats underwent partial hepatectomy and were divided into nonarterialization group and arterialization group. Dynamic observation of serum ALT and AST, and changes of ATP, ADP and AMP in hepatic tissues were determined at 2 h, 6h and 12h after operation. EC values were also calculated, and hepatic tissues were obtained for pathologic examination. Results （ 1 ） Compared to nonarterialization group, the serum AST and ALT of arterialization group showed no significant difference on the 2nd hour after the operation （ P 〉 0. 05 ） , but , serum AST and ALT were significantly decreased on the 12th hour in arterialization group （ P 〈 0. 01 and P 〈 0. 05 ） . （ 2 ） Compared with nonarterialization group, the ATP content of liver tissue and EC value were significantly increased on the 2 nd, 6 th and 12 th hour in arterialization group （ P 〈 0. 01 and P 〈 0. 05 ） . （ 3 ） Hepatic pathological changes were exacerbated progressively with the prolonged duration of ischemia in nonarterialization group , while the injury of hepatic cells in arterialization group was mild. Conclusions The partial arterialization of portal vein can lessen the damage of the liver after partial hepateetomy and transection of hepatic artery, and can improved the energy metabolism of the hepatic cells.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第1期23-25,共3页
China Journal of General Surgery
关键词
肝切除术
门静脉动脉化
Hepatectomy
Portal Vein Anteridization
作者简介
尹强，男，湖南衡阳人，中南大学湘雅二医院住院医师．主要从事肝胆胰外科疾病方面的研究。通讯作者：尹强 电话：13007314918（手机）；E-mail：qiangyin@hotmail.com.
