摘要
目的观察不同比例自异体微粒皮混合移植后创面愈合的效果。方法以雄性 Wistar大鼠为供体,在雌性SD大鼠背部建立全层皮肤缺损创面模型。将SD大鼠随机分为4组,每组 10只:(1)异体皮组,移植面积扩张比为10:3的异体微粒皮。(2)自体皮组,移植面积扩张比为10:1 的自体微粒皮。(3)混合1组,自异体微粒皮移植面积扩张比各为10:1。(4)混合2组,自异体微粒皮移植面积扩张比分别为10:1和10:3。于移植术后2、3、4周对各组大鼠创面进行外观和组织学观察,数码相机照相后运用图像分析软件测定创面愈合率和创面收缩率,并进行各组间的比较。结果 (1)异体皮组大鼠创面随着排斥反应发生,除创缘有新生表皮向内爬行外均为肉芽创面;自体皮组阅微粒皮数量偏少,术后2周仍有部分为肉芽创面;两个混合移植组术后2周创面基本上皮化。(2)移植后各组创面真皮内有不同程度的血管扩张和单个核细胞浸润,在异体皮组和混合2组中更加明显, 自体皮组及混合1、2组大鼠创面的表皮层明显增厚。(3)异体皮组移植后2-4周,随着排斥反应的发生,其创面愈合率明显下降。移植后3周,自体皮组创面愈合率为(55±26)％,明显低于混合1、2 组的(88±6)％和(76±10)％(P<0．05或0．01)。(4)移植后3周,混合2组创面收缩率为(69± 7)％,高于异体皮组[(58±11)％],其余各组之间比较差异无统计学意义(P>0．05)。结论适当比例的自异体微粒皮混合移植,可以促进创面愈合;当两者移植面积扩张比均为10:1时,具有较好的促创面愈合效果。
Objective To investigate the influence of different amount of allogeneic microskin in mixed grafting with certain quantity of autologous microskin on wound healing in rats. Methods Male Wistar rats served as alloskin donor rats. Forty female SD rats with full thickness skin defect were enrolled in the study, and they were randomly divided into four groups, i.e. group Ⅰ（ n = 10, with allogeneic microskin graft at area expansion rate of 10：3 ） ; group Ⅱ （ n = 10, with autologous microskin graft at area ex pansion rate of 10：1 ） ; group Ⅲ （ n = 10, with mixed grafting of autologous and allogeneic microskin at area expansion rate of 10： 1, respectively） ; group Ⅳ （ n = 10, with mixed grafting of autologous and allogeneic microskin at area expansion rate of 10：1 and 10： 3, respectively）. The wound healing rate, wound contraction rate and histological changes were observed at the 2, 3 and 4 post graft weeks （ PGW）. Results （ 1 ） In group I , there was mainly granulation tissue with some de novo epithelial cells appearing at the wound edge along with the rejection of grafted allogeneous skin in the rat wound. In group Ⅱ , there was still some granulation tissue remaining at 2 PGW due to insufficient amount of microskin. However, the wounds in the mixed grafting group appeared almost totally epithelialized. （2） Various amounts of mononuclear inflammatory cell infiltration and different degrees of angiectasis were observed in the dermal layer after the skin grafting in all groups, especially in group Ⅱ and Ⅳ. There was thickening of the epithelial layer in all groups except group Ⅰ. （3） The wound healing rate decreased obviously along with the development of rejection in group Ⅰ at 2 to 4 PGWs. The wound healing rate was （ 55 ± 26） % in group Ⅱ , which was obviously lower than that in group Ⅲ （88±6）% and in group Ⅳ （76±10）% at3 PGWs（ P 〈0.01）. （4） The contraction rate of the wound in group Ⅳ （69±7）% was much higher than that in group Ⅰ （58 ± 11）% at 3 PGWs（ P 〈 0.05） , and there was no difference among all the other groups. Conclusion Wound healing can be obviously accelerated by mixing some autologous microskin with appropriate amount of alloskin. Moreover, certain amout of autologous microskin （expansion rate 10：1）mised with the same proportion of allogeneie mieroskin seems to be more beneficial in promoting wound healing.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2006年第1期11-14,共4页
Chinese Journal of Burns
基金
国家重点基础研究发展计划资助项目(2005CB522605)第三军医大学第一附属医院临床研究专项基金资助项目(SW2004014)
关键词
移植
自体
移植
同种
创面愈合
Transplantation ,autologous
Transplantation,homologous
Wound healing
作者简介
通信（讯）作者：彭代智，Email：dzpeng@yahoo．com，电话：023-68754174
