摘要
目的 为角膜上皮损伤修复寻找新的有效方法。方法 运用MTT比色法 ,观察羊膜匀浆上清液在不同浓度及不同时相点 ,对兔角膜上皮细胞增殖的影响。运用活细胞计数方法检测兔角膜上皮细胞在不同羊膜匀浆上清液及不同时相点时的细胞活力。结果 羊膜匀浆上清液对兔角膜上皮细胞生长有促进作用 ,并呈剂量依赖性。结论 羊膜匀浆上清液可能能够应用于临床 ,促角膜上皮细胞修复。
Objective To select effective methods for accelerating corneal epithelial wound healing and treating in persistent corneal epithelial defect.Methods We observed the effects of supernatanta of homogenized fresh amnion at different concentration and at different hours on rabbit corneal epithelial cell by tetrazotium(MTT)colorimetric assay.The viability of rabbit corneal epithelial cell at different concentration of supernatanta of homogenized amnion was assessed by cell counting at different hours after culture.Results Supernatanta of homogenized amnion enhanced cellular proliferation effectively with a dose-dependent manner.Conclusion The results suggest that supernatanta of homogenized amnion can stimulate proliferation of human corneal epithelial cells and it may be of clinically potential use.
出处
《眼科新进展》
CAS
2004年第4期264-265,共2页
Recent Advances in Ophthalmology
关键词
角膜上皮细胞
羊膜匀浆
细胞培养
兔
corneal epithelial cell
amniotic homogenate
cell culture
rabbit
