摘要
刺五加(Acanthopanax senticosus(Rupr.EtMaxim.)Harms)是五加科植物,是全球畅销的植物药之一,含有大量生物活性物质,其中所含有的刺五加苷类具有抗疲劳、抗衰老、抑制肿瘤生长等作用,紫丁香苷则是其中的主要活性成分之一。用HPLC法测定黑龙江省不同地区刺五加中紫丁香苷的含量,确定了刺五加紫丁香苷用超声波提取1.5 h、60%甲醇作提取溶剂、提取温度为55℃的最佳提取条件组合。HPLC条件则是以检测波长为266 nm、流动相为甲醇/水(V/V)=28/72、流速为1 mL.m in-1、柱温为25℃。对黑龙江省不同地区类似生境条件下,同龄野生刺五加中紫丁香苷的含量进行比较,在所调查的7个地区中,以五营丰林国家自然保护区的生长在红松原始林下的刺五加紫丁香苷含量最高,五大连池雪洞风景区和牡丹江镜泊湖地区的刺五加紫丁香苷的含量最低。该研究结果对刺五加的采集和种植生境的选择具有积极的指导意义。
Acanthopanax senticosus （ Rupr. et Maxim） Harms belongs to Araliaceae, which is a popular plant medicine with lots of bioactive substance around the world. Eleutheroside has many functions, such as resisting weariness and senescence, restraining tumor growth etc. Syringin is a main component of eleutheroside. Ultrasonic extraction method with 1.5 hour, sixty percent carbinol liquor as extractant and 55℃ are the best combination of extraction method. HPLC suitable condition was 266 nm as measuring wavelenghth, carbinol and water（28：72） used as mobile phase, 1 mL ·min^-1 as velocity of flow, column temperature with 25℃. Syringin content in root and stem were compared and analyzed in similar habitats in Heilongjiang different zones. The results showed that in the seven investigation zones, syringin content of this species was highest in Wuying National Nature Reserve, which grew under Pinus koraiensis original forest, but lowest in Snow Hollow Beauty Spot of Wudalianchi and Miror Lake area of Mudan river. This result would be benefit on gathering many-prickle acanthopanax and selecting the cropping habitats.
出处
《植物研究》
CAS
CSCD
北大核心
2006年第1期123-126,共4页
Bulletin of Botanical Research
基金
哈尔滨市青年基金资助
关键词
刺五加
紫丁香苷
HPLC
Acanthopanax senticosus
syringin
HLPC
作者简介
张艳华（1967～），女，博士，副教授，从事植物学研究工作。
