摘要
目的:检测大肠癌中转录因子Ets-1,基质金属蛋白酶-1(MMP-1)和血管内皮生长因子(VEGF)的表达,探讨Ets-1在大肠癌血管生成和浸润转移中的作用.方法:应用免疫组化SP法检测61例大肠癌组织和21例正常大肠组织中Ets-1,MMP-1和VEGF蛋白的表达水平.结果:Ets-1,MMP-1和VEGF在正常大肠黏膜中表达均为阴性.在大肠癌组织中表达的阳性率分别为75.4%,78.7%和82.0%.其表达水平与肿瘤大小和分化程度无关(P>0.05),与Duke's分期(χ2=10.718,P<0.01;χ2=8.323,P<0.01;χ2=6.145,P<0.05)、浸润深度(χ2=7.705,P<0.01;χ2=19.101,P<0.01;χ2=14.707,P<0.01)、淋巴结转移(χ2=9.333,P<0.01;χ2=3.965,P<0.05;χ2=4.638,P<0.05)和远处转移(χ2=5.472,P<0.05;χ2=4.125,P<0.05;χ2=5.034,P<0.05)密切相关.在大肠癌中,Ets-1的表达与MMP-1和VEGF的表达呈正相关(r=0.447,P<0.01;r=0.425,P<0.05).结论:Ets-1在大肠癌中高表达,与临床分期、浸润深度、转移密切相关.Ets-1的表达与MMP-1和VEGF的表达呈正相关,三者的表达水平可作为判定大肠癌恶性生物学行为的参考指标.
AIM： To study the expression of E26 transformation- specific-1 （Ets-1）, matrix metalloproteinases-1 （MMP-1） and vascular endothelial growth factor （VEGF） in human colorectal carcinoma, and to explore the role of Ets-1 in the angiogenesis and metastasis of carcinoma. METHODS： The expression of Ets-1, MMP-1 and VEGF were detected in colorectal carcinoma （n= 61） and normal colon tissues （n = 21） by the immunohistochemical method respectively. RESULTS： Ets-1, MMP-1 and VEGF were negatively expressed in all normal mucosal tissues. The positive rates of Ets-1, MMP-1 and VEGF expression were 75.4%, 78.7% and 82.0% in colorectal carcinoma respectively. No significant correlation was found between their positive rates and tumor＇s size as well as the differentiation （P 〉0.05）, The expression of Ets-1, MMP-1 and VEGF were significantly correlated with Duke＇s staging （x^2 = 10.718, P 〈0.01; x^2 = 8,323, P 〈0.01; x^2 = 6,145, P 〈0,05）, the depth of invasion （x^2 = 7.705, P 〈0.01; x^2 = 19.101, P 〈0.01; x^2 = 14.707, P 〈0.01）, lymphatic invasion （x^2 = 9.333, P 〈0.01; x^2 = 3.965, P 〈0.05; x^2 = 4.638, P 〈0.05） and distant metastasis （x^2 = 5.472, P 〈0.05; x^2 = 4.125, P 〈0.05; x^2 = 5.034, P 〈0.05）. Ets-1 expression was positively associated with MMP-1 and VEGF level （r = 0.447, P 〈0.01 ; r = 0.425, P 〈0.05）. CONCLUSION： Ets-1 was over-expressed in colorectal carcinoma, and its expression was related to clinical staging, invasion and metastasis. Ets-1 expression was also positively related to MMP-1 and VEGF level. Their expression can become referential indexes to predict the malignant behavior of colorectal carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第20期2441-2445,共5页
World Chinese Journal of Digestology
作者简介
洪玮，女，1980-12-02生，安徽六安人，汉族，华中科技大学同济医学院消化内科在读硕士研究生，主要从事消化系肿瘤的研究 通讯作者：洪玮．430030，湖北省武汉市汉口解放大道1095号．华中科技大学同济医学院附属同济医院消化内科．hongwei_80@126 com电话：027-83663612 传真：027-83663585
