摘要
目的观察羟基脲(HU)对雄性大鼠的生殖毒性。方法雄性大鼠分别腹腔注射HU×100、200和400mg·kg-1,连续10d。末次给药后分别于d9、d23处死动物。结果停药d9时,200、400mg·kg-1组不活动精子增加明显(P<0.05或P<0.01);停药d23时,各给药组睾丸脏/体比都明显下降(P<0.05或P<0.01);400mg·kg-1组附睾脏/体比下降明显(P<0.05),并且仍然有大量不活动精子(P<0.01);200、400mg·kg-1组精子计数明显减少(P<0.01);随给药浓度增加,各组精子畸形数量增加(P<0.01)。结论雄性大鼠连续10d腹腔注射羟基脲100mg·kg-1以上,对生殖系统产生明显毒性。
AIM： To study the toxic effects of HU on reproductive system in male rats. METHOD： By intraperitoneal injection, HU were administrated to male Wistar rats at different doses of （ 100, 200, and 400 mg·kg^-1 ） once a day for 10 days, and the rats were randomly sacririced on the 9th and twentieth-third days after withdrawal of HU, respectively. RESULTS： Inactive sperm in 200 and 400 mg·kg^-1 group was higher than that in the control group on the 9th after withdrawal. On the twentieththird after withdrawal, the testis/body ratios, three treatment groups were decreased significantly than that in the control group （ P 〈 0.05, or P 〈 0.01）, the epididymides/ body ratios was decreased significantly in 400 mg·kg^-1 group, and inactive sperm remains had decreased significantly （ P 〈 0.01）, 200, 400 mg·kg^-1 groups of the number of sperm had decreased significantly than that in the control group （ P 〈 0.01）. In the groups treated, the rates of sperm deformity were significantly higher than that of the control, and the higher the expose concentration of HU, the higher the rate of sperm deformity on the 9th and the twentieth-third （ P 〈 0.01）. CONCLUSION： HU can result in the obviously adverse effects on the reproductive system of male rats, its aim organs may be testis and epididymis, and affect the course of spermatogenesis. After withdrawal, these damages are hard to recover and still development within a certain time.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第12期1330-1334,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家高新技术研究发展计划基金资助项目(863计划
№2002AA2Z342D)
作者简介
周莉，女，医师，博士研究生，研究方向：生殖毒理研究。Tel：010-66931632 E—mail：btzhl@tom．com
廖明阳，通讯作者，男，研究员，博士生导师，研究方向：药物毒理学和新药评价研究。Tel：010-66931628 E-mail：liaomingy@yahoo．com．cn
