摘要
采用nrDNA ITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNA ITS序列碱基差异值仅为0.002,nrDNA 18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NA ITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNA ITS更适合于该种属界定的分子标准.
Prorocentrum donghaiense and P. dentatum from CCMP were re-identified by two molecular makers-nrDNA ITS and 18S sequences. The sequences of these two species are very similar. The results show that the nrDNA ITS nucleotide divergence between P. donghaiense and P. dentatum CCMP is only 0. 002 and 18S nucleotide divergence is 0. 000. According to the molecular data, P. donghaiense and P. dentatum CCMP are a similar species. Other three nrDNA ITS sequences and eight 188 sequences of Prorocentrum were obtained from the Genbank. These sequences were analyzed and the phylogenetic trees were constructed by computer programme （Clustal X and Mega）. The results show that an nrDNA ITS region is a more suitable molecular maker to identifying interspecies of Prorocentrum and 18S sequence is too conservative.
出处
《海洋学报》
CAS
CSCD
北大核心
2006年第1期127-131,共5页
基金
国家重点基础研究发展规划资助项目(2001CB409701)
作者简介
罗立明（1976-）。女，广西壮族自治区河池市人，助理研究员，从事藻类学研究，Email：llm@rose．whiob．ac．cn
通讯作者，E—mail：hongjun@rose．whiob．ac．cn
