摘要
对五株赤潮异弯藻核糖体转录单元内间隔区(ITS区)进行了PCR扩增和序列测定,并结合GenBank中收录的所有赤潮异弯藻ITS序列信息,比较了它们的遗传距离和相似系数,同时结合GenBank中发布的针胞藻纲其他三个属的代表种ITS序列信息,采用邻接法(neighbour-joining,NJ)和最小进化法(minimum evolution,ME)构建系统发育树。结果表明,五株赤潮异弯藻ITS全序列总长度及碱基组成完全一致,均为538 bp,与GenBank中收录的四株赤潮异弯藻仅有一个碱基不同,遗传距离和相似系数分别为0.002和0.980,而与其他赤潮异弯藻ITS序列完全相同,遗传距离和相似系数分别为0.000和1.000。用两种方法构建的系统发育树结构完全一致,赤潮异弯藻属与海洋卡盾藻属亲缘关系较近,而与另外两属的亲缘关系较远。
The internal transcribed spacer （ITS） region of five strains which collected from Norway,English Channel, New Zealand, U. S. and Qingdao（China）was amplified with polymerase chain-reaction （PCR） and sequenced. Pairwise distance and percent identity between ITS sequence of the 5 strains and others retrieved from GenBank were ccalculated. The phylogenetic trees（NJ and ME） were constructed with the ITS sequences which linked to other Raphidophyceae. Results showed that the sequence of 5 strains was identical, which was 538 bp, only one base pair different from other four strains saved in GenBank. Pairwise distance and percent identity are 0. 002 and 0. 098, respectively. Otherwise completed identical sequences had been found when compared with other strains. The phylogenetic trees constructed by two methods showed totally same results. The Heterosigma had a close relationship with Chattonella, while was distant from other two classes.
出处
《海洋学报》
CAS
CSCD
北大核心
2008年第5期107-112,共6页
基金
国家海洋局近岸海域生态环境重点实验室基金资助项目(200504)
关键词
赤潮异弯藻
ITS
分子鉴定
Heterosigma akshiwo
ITS
molecular identification
作者简介
梁斌（1979-），男，河北省沧州市人，助理研究员，硕士，海洋生物学专业。E-mail：bliang@nmemc．gov．cn
