摘要
目的探讨针刺治疗血管性痴呆的作用机理。方法通过栓子注入法制作多发梗塞性痴呆模型,随机分为模型组、针刺组和非穴组,并设正常组和假手术组。应用原位杂交和免疫组化的方法检测痴呆大鼠海马区CuZnSOD的基因表达情况,并分析针刺的干预作用。结果与正常组比较,模型组大鼠海马区CuZnSOD mRNA及蛋白表达下降(P<0·01);针刺组可明显增强其表达水平(P<0·01),与正常组及假手术组比较无显著性差异。结论针刺在转录和翻译水平上均可上调CuZnSOD的表达水平,从而增强抗氧化酶活性,以有效地清除自由基,改善痴呆大鼠的智能状态。
Objective To explore the mechanism of acupuncture in treatment of multi-infarct dementia （MID）. Methods The multi-infarct dementia model was made by injection of emboli into the internal carotid artery. The dementia rats were randomly divided into 3 groups model group, acupuncture group and non-acupoint acupuncture group, and a normal group and a sham operation group were set up. The expression of CuZnSOD in the hippocampus of the rats were detected by in situ hybridization and immunohistochemical assay and effects of acupuncture were analyzed. Results The expressions of mRNA and protein of CuZnSOD in the hippocampus of the model group were significantly attenuated as compared with that in the normal control group. Both the parameters above were significantly increased in the acupuncture group as compared with the model control group, but with no significant difference between the acupuncture group and the normal control group. Concision Acupuncture can up-regulate the expressions of mRNA and protein of CuZnSOD in the rat with MID, so as to strengthen activity of antioxidase and effectively remove free radicals and improve intelligence of MID rats.
出处
《中国针灸》
CAS
CSCD
北大核心
2006年第2期129-132,共4页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金:30472242
天津市科技发展计划重点项目:05YFSYSF01500
作者简介
刘存志（1975-），男，医学博士，主治医师。研究方向：针刺防治脑血管疾病、老年期痴呆的基础和临床研究。e-mail：lcz623780@126．com
