摘要
为了提高在一般培养条件下不易产生分生孢子的黑麦麦角菌(Claviceps purpurea)的产碱率,对原生质体诱变的可行性进行了探讨。实验表明,对α-麦角隐亭(α-Ergokryptine)产生菌的原生质体采用紫外线(UV)照射9分钟,γ-射线(^(60)Co)10万伦琴/20分钟辐射,亚硝基胍(NTG)6mg/ml 60分钟进行诱变处理,均可获得较稳定的高产突变株。经过上述三种方法的交替诱变处理,α-麦角隐亭的产率由原始菌株的40mg/L,提高到524mg/L,增加了12倍,说明用原生质体进行诱变处理,配合田间复壮,对提高C.purpurea α-麦角隐亭的产率是可行的。
The feasibility of the protoplast mutagenesis for increasing α - ergokryptine productivity was studied on a Claviceps purpurea strain, which was not easy to produce conidia under laboratory conditions. The experiments showed that the stable mutants with increased titers were obtained by UV-irradiation, γ-ray(60Co) radiation or NTG treatment of the protoplasts with the appropriate dosages. After mutagenesis by methods mentioned above and selection the alkaloid productivity was increased by 13 times, from the initial titers 40 mg / L to 524 mg / L . The results indicate that the protoplast mutagenesis combining with field rejuvenation is a feasible approach to increase α- ergokryptine yield of the C. purpurea strain.
出处
《真菌学报》
CSCD
北大核心
1996年第3期215-219,共5页
基金
中国医学科学院
中国协和医科大学科学基金
关键词
麦角菌
α-麦角隐亭
原生质体
诱变育种
Claviceps purpurea, Protoplast, Mutagenesis, α- Ergokryptine.
