摘要
目的提取补阳还五汤中的多糖并测定其含量。方法用微波辅助法提取,料液比为1∶7,提取时间20min,微波功率325w。用蒽酮比色法测定多糖的含量,加蒽酮试剂沸水浴10 min,检测波长λ=620 nm,回归方程A=-0.044 4+0.001 3X,r=0.999 54。结果补阳还伍汤中多糖的提取率为7.75%。结论微波辅助萃取大大缩短了提取时间,提高了收率,多糖的测定方法简便易行,准确可靠。
Objective To extract the polysaccharides Methods The polysaccharides of Buyang Huanwu Tang and determine content in Buyang Huanwu Tang. were extracted by microwave assisted extraction （MAE）. The water volume was 7 times of materals and extraction time was 20 minute with microwave power of 325w. The concentration of polysaccharides was dertermined by anthracenone colorimetry. Anthracenone reagent was added in boiling water , bathing for 10min , detection wavelength was at 620 nm, the regression equation Y=-0. 044 4＋0. 001 3X,r=0. 999 5. Results The extraction rate of polysaccharides in Buyang Huanwu Tang was 7.75%. Conclusion MAE shortens the extract time greatly and enhances the yield obviously. The method was convenient and feasible for determination of polysaccharides in the preparation.
出处
《现代中药研究与实践》
CAS
2005年第6期38-40,共3页
Research and Practice on Chinese Medicines
关键词
补阳还伍汤
多糖
微波辅助萃取
蒽酮比色法
正交实验
Buyang Huanwu Tang
microwave assisted extraction （MAE）
polysaccharide
anthracenone colorimetry
orthogonal design
