摘要
采用硅胶柱层析方法,流动相分别为氯仿-甲醇-水(65∶35∶10,V/V)、氯仿-甲醇-水(6∶4∶1)和正丁醇-乙酸乙脂-甲醇-水(4∶2∶1∶1)和高效液相(Nuc leosil C18(250 mm×10 mm,5μm)色谱柱;流动相为己腈-水(75∶25);流速为3.0 mL/m in;柱温30℃;检测波长203 nm。从中国鲜人参中分离得到丙二酰基人参皂苷R c和丙二酰基人参皂苷Rb2,并通过其理化性质、红外光谱、质谱和核磁共振谱对化合物的结构进行了鉴定,证明其结构分别为3-O-[6-O-丙二单酰-β-D-葡萄吡喃糖(1→2)-β-D-葡萄吡喃糖]-20-O-[α-L-阿拉伯呋喃糖(1→6)-β-D-葡萄吡喃糖]-20(S)-原人参二醇和3-O-[6-O-丙二单酰-β-D-葡萄吡喃糖(1→2)-β-D-葡萄吡喃糖]-20-O-[α-L-阿拉伯吡喃糖(1→6)-β-D-葡萄吡喃糖]-20(S)-原人参二醇;丙二酰基人参皂苷R c为首次从中国鲜人参中分得。
Malonyl-ginsenosideRc and Malonyl-ginsenoside Rb2 were isolated from the fresh mot of Panax ginseng by column chromatography on silica gel eluting with CHClJMeOH/H2O（65：35：10, V/V）, CHCl3/ MeOH/H2O（6：4： 1, V/V）, n-BuOH/EtOAc/MeOH/H2O （4： 2： 1： 1, V/V） separately and high performance liquid chromatography. The chromatographic conditions were as follows： a Nucleosil C18 （250 mm × 10 mm, 5μm） column was eluted with acetonitrile/water （75：25）as mobile phase at the column temperature of 30℃, βow rate of 3.0 mL/min and ultraviolet detection at 203 nm. On the basis of chemical, infrared spectra, mass spectrum and nuclear magnetic resonance evidence, two of the compounds have been elucidated as 3：O-[6-O- malonyl-β-D-glucopyranosyl （ 1 →2 ） -β-D-glucopyranosyl ] -20-0-[ α-L-arabinofuranosyl （ 1 →6 ） -β-D-glucopyr- anosyl ] -20 （ S ） -protopanaxadiol, and 3-O- [ 6-O-malonyl -β-D- glucopyranosyl- （ 1 →2 ） -β-D-glucopyranosyl ] - 20-0-[ α-L-arabinopyranosyl （ 1 →6 ） -β-D- glucopyranosyl ] -20 （ S ） -protopanaxadiol. Malonyl-ginsenosid e-Rc was isolated at first time from the root of Panax ginseng cultivated in China.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第12期1783-1786,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No.30370159)
