摘要
目的:观察高渗氯化钠羟乙基淀粉40注射液(HSH)对失血性休克／内毒素二次打击大鼠复苏时是否能降低氧自由基的 产生。方法:SD大鼠54只,采用失血性休克／内毒素二次打击模型。动物随机分为对照组(C组)、乳酸林格氏液复苏组 (RL组)、高渗氯化钠羟乙基淀粉40注射液(HSH组)。检测肠组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量;肠标 本进行病理学检查。结果:休克复苏后1h,RL组与HSH组肠组织MDA含量均明显升高,SOD活力明显下降;复苏后2h, HSH组肠组织MDA含量开始下降,SOD活力升高,而RL组变化不明显;至复苏后4h,两组比较MDA和SOD相差显著(P <0．05);病理学检查,HSH组肠组织较RL组损伤轻。结论:HSH可以降低机体氧自由基的产生,减轻肠组织缺血再灌注损 伤。
Objective：To observe whether hypertonic solution chloride hydroxyethyl starch 40 injection （HSH） reducing oxygen free radicals of rat after resuscitation from hemorrhagic shock and intratracheal lipopolysaccharide （LPS）.Methods：Fifty-four SD rats were randomly divided into group C group RL and group HSH.Each group was divided into three groups according to the time （hours） afer resuscitation （group C1 group C2 ,group C4 group RL1, group RL2, group RL4 group HSH , group HSH2, group HSH4）. Hemorrhagic shock and intratracheal LPS animal model was prepared. The levels of superxide dismutase （SOD）, molondialdehyde （MDA） were determined Samples of intestine were removed for histologic analysis. Results：1h after the resuscitation, significantly increased MDA levels and reduced SOD levels were detected in group RL and group HSH,2h after the resuscitation MDA levels began to decline and SOD levels rise in group HSH.But there was no obvious change in group RL.4h after the resuscitation,there was statistically significant difference between group RL and group HSH about MDA and SOD levels （P〈0. 05）.The histologic analysis showed that intestine injury slighter in group HSH than that in group RL.Conclusion：HSH can effectively reduce oxygen free radicals levels in intestinedecrease tissue ischemia/reperfusion injury.
出处
《中国医药导刊》
2005年第6期451-453,共3页
Chinese Journal of Medicinal Guide
