摘要
目的:从苦瓜中分离水溶性苦瓜多糖,并研究其组成性质。方法:采用DEAE纤维素柱层析和凝胶柱层析分离纯化得到苦瓜多糖MCP2和MCP4,SepharoseCL-6B凝胶柱层析和醋酸纤维膜电泳检测证明为均一组分。气相色谱法和薄层层析法测定其多糖组成,凝胶过滤法测定其相对分子质量,红外光谱测定其糖苷键类型。结果:苦瓜多糖MCP2为半乳聚糖,MCP4含有鼠李糖、木糖、半乳糖和阿拉伯糖,它们的摩尔比为1:0.75:1.83:0.85。平均相对分子质量分别为5.37×104,9.65×104。它们的红外光谱中有典型的多糖吸收峰,紫外扫描无核酸和蛋白特征吸收峰。结论:MCP2,MCP4首次从苦瓜中提取获得,为均一多糖组分。
Objective： To study the isolation, purification and composition of Momordica charantia L. polysaccharide（MC）. Methods MC was purified by DEAE ion-exchange cellulose （DEAE-52） and Sephadex G-100.The components of the saccharide were determined by GC and TLC. Its MW was measured by gel filtration. The form of glucosidic bond was determined by IR. Results： Rhamnose, xylose galactose, and arabinose were found in MCP4 with the molar ratio of 1：0.75：1.83：0.85 while galactose was found in MCP2, Their average MWs were estimated respectively to be 5.37 × 10^4 and 9.65 ± 10^4. Typical absorption of polysaccharide was shown in their IR spectrum. They had no typical absorption of nucleic acid or protein by UV scanning. Conclusion： MCP2 and MCP4 are first isolated from Momordica charantia L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第11期115-119,共5页
Food Science
基金
江苏省科技攻关项目(BE2004360)
关键词
苦瓜
多糖
分离
纯化
组成
Momordica charantia L.
polysaccharide
isolation
purification
composition
作者简介
董英（1954-），女，教授，博导，研究方向为食品生物技术。
