摘要
目的:利用磁共振ADC值测量与HSP 70免疫组化检测技术,研究新生猪急性期重度缺氧缺血性脑病(HIE)模型脑部可逆缺血区的存在与否及其演变规律.方法:7日龄健康新生约克猪45只随机分为正常对照组(5只)和HIE组(40只).HIE组于缺氧缺血后24h内不同时间点采集ADC值与HSP 70资料.ADC值测量与HSP 70阳性细胞计数采用ADC值最低的区域至ADC值正常区域等距4分区法.结果:HIE组Ⅰ区ADC值下降的幅度最大,随时间呈先降后升的变化规律,HSP 70阳性细胞计数随时间未见明显变化,病变趋向坏死;Ⅱ区、Ⅲ区ADC值下降的幅度依次递减并随时间逐渐上升;Ⅳ区ADC值保持正常水平;Ⅱ～Ⅳ区HSP 70阳性细胞计数均随时间明显增加,病变趋向可逆.结论:HIE病理演变过程中的ADC值与HSP 70表达之间具有很好的一致性,ADC值变化具有鉴别HIE过程中脑组织坏死和可逆脑缺血损伤的能力.
Objective：To study reversible cerebral ischemia regions of acute severe hypoxic ischemic encephalopathy （HIE） in piglet model by MRI ADC values and HSP 70. Methods：Seven days old piglets （n=45） were divided into 2 groups at random ：group A （normal control group, n= 5 ） and group B （HIE group, n= 40, produced by bilateral common carotid artery occlusion and then inspiration of 4% oxygen about 1 hour）. On ADC maps,4 regions of interest （ROD were set from the minimum ADC values point to the normal ADC values area, and named ROI Ⅰ , ROI Ⅱ , ROI Ⅲ , ROI PC in order. The data of ADC values and HSP 70 positive cells were acquired at the time point of 0,1,3,6,12,18 and 24h after formation of hypoxie isehemia. Results： In HIE group, the ADC values of ROI Ⅰ dropped first and then rose, and it dropped most seriously at 12～18h after formation of hypoxie isehemia. The one of ROI Ⅱ and ROI Ⅲ dropped less seriously when compared with ROI Ⅰ and rose gradually just after formation of hypoxie isehemia. The one of ROI IV was normal. The amount of HSP 70 positive cells in ROI Ⅰ was small and it did not increase with the time passing on. The amount of HSP 70 positive cells in ROI Ⅱ～Ⅳ was large and it increased obviously with the time passing on. Conclusion： Consistent with the HSP 70,the ADC values can make differentiation between reversible and irreversible cerebral ischemia regions in HIE.
出处
《放射学实践》
2005年第11期941-944,共4页
Radiologic Practice
关键词
缺氧缺血
脑
模型
动物
磁共振成像
表观扩散系数
热休克蛋白70
Hypoxia-ischemia,brain
Models,animal
Magnetic resonance imaging
Apparent diffusion coefficient
Heat shock protein 70
作者简介
郭岳霖（1976-），男，广东潮州人，硕士，住院医师，主要从事神经系统影像学诊断工作。
