摘要
目的：通过比较不同极性溶媒瑞香狼毒提取物的体外抗肿瘤活性，确定瑞香狼毒抗肿瘤活性成分所在的极性区间。方法：采用索氏回流提取法，依次用石油醚、乙酸乙酯、丙酮和乙醇对狼毒进行回流提取，并用不同浓度的各溶剂提取物分别处理Eca109、K562、HepG_2细胞株，通过MTT检测法，比较各溶媒提取物对肿瘤细胞的抑制活性。结果：石油醚提取物和乙酸乙酯提取物对体外培养肿瘤细胞表现出较强的抑制作用，在测定浓度范围内呈现出良好剂量依赖性，石油醚提取物最大抑制率分别为78％(Eca-109)、93％(K562)、95％(HepG_2)，乙酸乙酯提取物最大抑制率分别为53％(Eca-109)、91％(K562)、87％(HepG_2)；而丙酮和乙醇提取部位在测定浓度范围内则显示出很弱的抑制作用；在各提取物中石油醚提取物的体外抗肿瘤作用最强。结论：石油醚提取成分是瑞香狼毒中体外抗肿瘤活性最强的部位。
AIM： To find the optimal polarity zone by comparing the antitumor effects of various polarity solvent extracts. METHODS： The various polarity solvent was used to separate the plant dry powder （petroleum ether, ethyl acetate, acetone, and ethanol）. The antitumor activities of the different extracts on human cancer cell lines in vitro were analyzed using MTT assay. RESULTS： The petroleum ether extract and the ethyl acetate extract strongly inhibited the proliferation of leukemia K562 cells,oesophagus cancer Eca-109 cells and hepatocarcinoma HepG2 cells, and showed significant dose-dependent response while the acetone extract and the ethanol extract exhibited low antitumor activities in vitro. The petroleum ether extract was the most active part. CONCLUSION： The petroleum ether extract is the principal antitumor extract from SteUera chanraejasmel L.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第10期1140-1142,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
瑞香狼毒
不同极性溶媒
MTT
抗肿瘤活性
石油醚提取物
Stellera chamaejasmel
various polarity solvent extract
MTT
antitumor activity
petroleum ether extract
作者简介
罗慧英,女,在读博士,研究方向：植物活性成分的研究. Tel： 13309483290 E-mail： louria_luo@sina.com
王爱勤,通讯作者,男,研究员,博士生导师,研究方向：有机合成与功能材料. Tel： 0931-4968118 E-mail： aqwang@lzb.ac.cn
