摘要
目的探讨房水和血清中神经元特异性烯醇化酶（或称γ－烯醇化酶）同工酶（ｎｅｕｒｏｎ－ｓｐｅ－ｃｉｆｉｃｅｎｏｌａｓｅ，ＮＳＥ）测定在视网膜母细胞瘤中的诊断价值。方法应用醋酸纤维膜板快速电泳法分析２３例（房水２１例，血清２３例）视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，Ｒｂ）及２５例（房水２１例，血清２５例）对照组房水和血清烯醇化酶同工酶，并用荧光箔层扫描仪测定其同工酶的相对活性及其所占百分比。结果Ｒｂ患眼房水全部发现αα、αγ、γγ３种烯醇化酶同工酶呈强阳性的荧光带，ＮＳＥ阳性检出率为１００％，ＮＳＥ相对活性占３种烯醇化酶同工酶总相对活性的百分比为４５％±９％；而对照组患眼房水，仅在２例外伤性前房积血的血性房水中发现αα及较弱αγ、γγ荧光带，在白内障、青光眼及４例Ｃｏａｔｓ病患者中，未发现任何荧光带。２３例Ｒｂ患者血清中，有１８例发现α、αγ和／或γγ荧光带，ＮＳＥ阳性检出率为７８．２％；５例仅见αα带，其ＮＳＥ相对活性强度及其占总相对活性百分比为３６％±９％，均较房水明显。在对照组血清中，仅见αα带，ＮＳＥ阴性率为１００％。结论视网膜母细胞瘤房水和血清中ＮＳＥ显增加，免疫电泳测定对视网膜母细胞瘤具有诊?
ObjectiveNeuron-specificenolase(NSE)orisoenzymescontainingγ-enolasearecon-sideredvaluableinthediagnosisoftumorsofneuroectodermalorigin.MethodWeusedrapidelec-trophoreticmethodonceluloseacetateplatetodeterminethepaternsofenolaseisoenzymesinthe21aqueoushumorand23serumspecimensfrom23patientswithretinoblastoma(Rb)and21aqueousand25serumspecimensfrom25controlcasestoevaluateNSEinthediagnosisofRb.Theassayalowedas-sessmentofalthreemajorisoenzymes(αα,αγandγγ),andNSErelativeactivityanditspercentageinthetotalrelativeactivityofthethreeenolaseisoenzymeswereassessedbymeansofafluorometer.Re-sultsAqueousfromalpatientswithRbcontainedαα,αγandγγisoenzymesandpresentedstrongpositive,thepositiverateofNSEbeing100%anditsrelativeactivityacountingfor45±9%oftheto-talrelativeactivityofthe3enolaseisoenzymes;noenolasewasdetectableinthecontrolaqueouswithcataract,glaucomaandCoats'sdiseases(4cases),butintwopatientswithtraumatichyphemaααandfaintαγandγγpresentedintheaqueous.Thecontrolserumcontainedonlyanααband,whiletheserafrompatientswithRbcontainedαα,αγandγγbandsin18of23specimens,thepositiveratebeing78.2%,andonlyααbandsintherest5specimens(21.8%).InthepatientswithRb,theNSErelativeac-tivityanditspercentageinthetotalrelativeactivityofthe3isoenzymesinserum(36±9%)weresig-nificantlylowerthanthatinaqueous.ConclusionTheamountsofNSEincreasesignificantlyinbothserumandaqueousfrompatientswithRb,andtheimmunoelectrophoreticassayfordeterminationofenolasepaternsisvaluableinthediagnosisanddiferentialdiagnosisofRb.Inaddition,themethodisrapid,simpleandrequiresonlyalitleamount(<5μl)ofsample.
出处
《中华眼科杂志》
CSCD
北大核心
1996年第3期219-223,共5页
Chinese Journal of Ophthalmology
基金
广东省卫生厅科研基金
关键词
视网膜母细胞瘤
诊断
房水
神经元
烯醇化酶
AqueoushumorSerumNeuron-specificenolaseRetinoblastomaImmu-noelectrophoresis
