摘要
目的:观察IL-1β和TNF-α对培养的人RPE细胞FasL蛋白、mRNA表达的影响。方法:流式细胞仪检测暴露于IL-1β和/或TNF-α的RPE细胞FasL的表达,并用mRNA斑点杂交分析mRNA的改变。结果:流式细胞仪显示23.4%培养的RPE细胞表达FasL。IL-1β,TNF-α和二者联合应用后FasL阳性率则分别为25.7%,9.7%和17.2%。mRNA斑点杂交亦证实TNF-α下调FasLmRNA的表达。结论:TNF-α可下调体外培养的人RPE细胞FasL蛋白,mRNA的表达。
AIM： To observe the effect of IL-1β and TNF-α on FasL protein and mRNA expression of cultured human RPE cells. METHODS： FasL expression of human RPE cells exposed to IL-1β and/or TNF-α was detected by flow cytometry. FasL mRNA expression was analyzed by dot-blot hybridization. RESULTS： FasL was expressed on 23.4% of cultured human RPE cells as shown by flow cytometry. While IL-1β, TNF-α, and both were added to the cultured media, the expression of FasL-positive cells was 25.7%, 9.7%, and 17.2%, respectively. Dot-blot hybridization for mRNA confirmed that FasL mRNA expression was downregulated by TNF-α, CONCLUSION： TNF-α can downregulate FasL protein and mRNA expression in cultured human RPE cells.
出处
《国际眼科杂志》
CAS
2005年第5期916-918,共3页
International Eye Science
作者简介
刘兵,1990年毕业于中国医科大学,1996年毕业于西安医科大学,眼科硕士学位;1999年毕业于第四军医大学,博士学位,副主任医师,研究方向：屈光和眼底病.通讯作者：liubing20@yahoo.com.cn
