摘要
目的:探讨EB病毒DNA(EBV-DNA)在鼻咽癌放疗前后的动态变化及与复发、远处转移的关系。方法:采用PCR加限制性内切酶酶切技术检测EBV-DNA。结果:放疗前,74例标本中有71例(95.9%)EBV-DNA片段检出阳性;放疗50 Gy/5周时,23例鼻咽原发灶和颈部淋巴结消失,其阳性率为13.0%(3/23),余51例肿块未消者阳性率为62.7%(32/51);放疗至70 Gy/7周时,7例放疗后有残留,有残留者在放疗结束时EBV-DNA片段的检出阳性率为71.4%(5/7),在67例肿块消失者中未检出阳性EBV-DNA片段。12例复发者中,11例EBV-DNA片段检出阳性;8例转移者中EBV-DNA片段检出均为阳性。结论:检测血浆EBV-DNA能很好地反映肿瘤的消长,是诊断鼻咽癌残留、复发及远处转移的敏感指标。
Objective：To study the varies of blood plasm Epstein-Barr virus（EBV）DNA before and after radiotherapy in nasopharyngeal carcinoma and the relationship between it with the tumor growth and decline, residual and relapse as well as metastasis. Method：Polymerase chain reaction（PCR）with restriction enzyme technique was used to detect EBV-DNA. Result： Seventy-one out of 74 specimens were EBV DNA positive, the positive rate was 95.9%（71/74）. In the time of radiation dose 50 Gy/5 week, the primary nasopharynx tumors and lymph node of neck in 23 cases disappeared, the positive rate was l 3.0 % （3/23）. In the group of tumors remained, the positive rate was 62.7 % （32/51）. In the time of radiation dose 70 Gy/7 week, 7 out of 74 existed residual tumor after radiotherapy,5 out of 7 cases were positive,the positive rate was 71.4%（5/7）. In the cases the tumors disappeared,EBV- DNA was negative. Eleven out of 12 cases with relapse after radiotherapy EBV-DNA fragments detected were positive. All of 8 patients with metastasis after radiotherapy, their EBV-DNA fragments detected were positive. Conclusion：The detection of blood plasm EBV DNA can reflect the tumors growth and decline well. It is an important and sensitive index in diagnosing the residual and relapse as well as the metastasis of nasooharyngeal carcinoma.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第20期920-922,共3页
Journal of Clinical Otorhinolaryngology
基金
湖南省自然科学基金资助项目(No:03jjy3058)
作者简介
通讯作者：姜武忠（Email：jwz903@sohu．com）.
