摘要
目的:对三七叶中的2种主要黄酮类成分山柰酚-3-O-β-D-半乳糖(2→1)葡萄糖苷(Ⅰ)与槲皮素-3-O-β-D-半乳糖(2→1)葡萄糖苷(Ⅱ)进行含量测定。方法:采用反相高效液相色谱法,使用 ZORBAX Extend C_(18)柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.15%乙酸水溶液(35:65),流速1.2 mL·min^(-1),检测波长268 nm,柱温25℃。结果:山柰酚-3-O-β-D-半乳糖(2→1)葡萄糖苷与槲皮素-3-O-β-D-半乳糖(2→1)葡萄糖苷在进样量0.5-20μg范围内均有良好的线性关系,相关系数 r 皆为0.9999;Ⅰ的平均加样回收率为97.5%,RSD 为0.78%;Ⅱ的平均加样回收率为101.8%,RSD 为1.1%。结论:方法简便快捷,准确度高。
Objective：To determine main flavonoids Ⅰ kaempferol 3 - O - （2＂ - β - D - glucopyranosyl） - β - D - galactopyranoside and Ⅱ quercetin 3 - O - （ 2＂ - β - D - glucopyranosyl ） - β - D - galactopyranoside ） in the leaves ofpanax notoginseng by RP- HPLC. Methods：The separation was performed on ZORBAX Extend C18 column（250mm×4.6mm,5μm）,using 35% methanol and 65% water/acetic acid mixture（ 100/0.15 v/v）as mobile phase with the following rate of 1.2 mL·min^-1 at 25 ℃, the wavelength for measurement was 268 nm. Resuits： The calibration curves were linear in the range of 0. 5 - 20μg for compounds Ⅰ （ r = 0. 9999, n = 6 ） and Ⅱ （ r =0. 9999,n =6）. The average recoveries of compound Ⅰ was 97.5% （ RSD =0. 78% ） ,and that of compound Ⅱ was 101.8% （RSD=1.10% ）. Conclusion：This method is accurate and reproducible.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第9期1089-1091,共3页
Chinese Journal of Pharmaceutical Analysis
作者简介
通讯作者（corresponding author）
