摘要
用醇提取和溶剂分离法从瑞香狼毒根中提得瑞香狼毒总黄酮进行动物毒性试验，结果表明，小白鼠灌胃ＬＤ＿（５０）为５２４．８１ｍｇ／ｋｇ；家兔耳静脉注射７５ｍｇ／ｇ后主要表现角弓反张，痉挛抽搐，呼吸困难，心电图显示心力衰竭印象；麻醉家兔耳静脉注射２５ｍｇ／ｋｇ后出现呼吸、脉搏减慢及短暂的脉压增大，随后脉压消失；家兔灌胃７００ｍｇ／ｋｇ后出现腹痛、腹泻和体温下降，血清ＧＰＴ、ＧＯＴ、ＭＰ活性２４ｈ内无变化，而ＢＵＮ含量显著升高，病理变化以各脏器瘀血、胃肠道出血、肺气肿及心肌纤维、肝细胞和肾小管上皮细胞颗粒变性为特性；对象兔离体肠道运动有抑制；皮肤原发刺激指数为２．８９，属中等刺激物。
otal-flavonoid with flavonoid compound content
of 22.72%was ob-tained from the roots of S camaejasme L.by means of ethanol extraction and
solvent process.The total-flavonoid toxicity experiments indicated that the LD50 in mice given
orally was 524.81 mg/kg,rabbits poisoned by intravenous injection at dosage of 75 mg/kg
showed the symptoms of opisthotonus,spasm,dyspnea,and heart failtire in ECG;the
anesthetized rabbits given by inter-venous injection at the dosage of 25 mg/kg showed the
symptoms of reduced respiratory and pulse rate,transiently increased pulse pressure and then
disappearance;rabbits given orally at the dosage of 700 mg/kg showed the symptoms of
abdominal pain,diarrhea,and body temperature decline;the activities of serum GPT,GOT,AKP
had no change,but concentration of BUN elevated signifiently in 24 h;pathological finding
characterised by intensive extravasation of viscera,gastroen-teric
heamorrhage,pneumonectasis and the granular degeneration of hepatocytes,epithe-lial cells of
renal tubules and cardiac muscle,the automatic contraction of extracorporeal small intestine of
rabbits could be inhibited,primary stimulative of skin was 2.89,be-longing to a middle
stimulant.
出处
《西北农业大学学报》
CSCD
1996年第4期35-38,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
关键词
瑞香狼毒
黄酮
小白鼠
家兔
中毒
Stellera chamaejasme L.,total-flavonoid,mice,rabbit,toxicity
