摘要
研究了两种不同的电泳缓冲系统及染色方法对水稻酯酶同工酶的鉴定效果.结果表明：Tris-HCl系统的分辩率比Tris-citric系统的高.分析了杂交水稻不同发育时期材料、不同取材部位酯酶同工酶的表达.结果表明：3～4 d芽龄的幼苗为最适宜的鉴定时期,取材部位不影响标记酶带的表达.应用该方法对供试材料的酶谱类型进行了划分：①偏亲型（汕优559、协优57、协优559、65002）;②互补型（特优559、特优多C 1号）;③杂合型（汕优63、协优63、特优63）.其中互补型在种子真实性和纯度鉴定中具有一定利用价值.用互补型对生产用种进行检测,并与相应的大田鉴定结果进行回归分析,表明田检纯度和酶谱纯度呈极显著正相关.
Two buffer systems of polyacrylamide gel electrophoresis（PAGE） followed by two staining methods were used for rice variety identification,The resultant banding patterns indicated that Tris-HCl system showed the better resolution than Tris-citric system. The most suitable period for identification was the 3 rd or 4 th seedling. Extracting esterase from the different part of seedlings did not affect the express of the marker patterns. By this method, the esterase banding patterns of all materiales may be classified into three types：（1）similar to parent marker patterns （ shanyou 559, Xieyou 57, Xieyou 559 and 65002）, （2） coexisting two parental marker patterns （Teyou 559 and Teyoudou C 1 ） ,（3）mixed type （ Shan you 63, Xieyou 63 and Teyou 63 ）. Among the three types, coexisting type proved to be the most valuable. The zymogram purity of popularized seeds were tested, and compared with the variety purity in field. The result showed that the correlation coefficients between them were highly significant .
出处
《种子》
CSCD
北大核心
2007年第2期28-32,共5页
Seed
基金
安徽省"十五"优秀人才基金资助。
作者简介
陶芳（1970-）,女;硕士,讲师,在读博士研究生; E-mail：taofang007@126.com.
通讯作者：姚大年（1955-）,男;E-mail：dnyao@163.com.
