摘要
使用高效液相色谱法，酸滴定法，气相色谱法从膜磷脂含量、磷脂酶Ａ２（ＰＬＡ２）活性及花生四烯酸（ＡＡ）含量三个方面研究了体温过高大鼠肝细胞膜磷脂代谢的变化；用放射配基结合分析法测定了β肾上腺素受体的变化；同时观察了预先使用ＰＬＡ２抑制剂米帕林对这些变化的调节作用。结果表明，体温过高大鼠肝细胞膜磷脂分解代谢加强；β肾上腺素受体的最大结合容量（Ｂｍａｘ）和解离常数（ｋｄ）值也都明显减少。而预先使用米帕林的大鼠，除β肾上腺素受体的Ｂｍａｘ进一步下降外，其余所测各项指标与对照值无明显差异。
Effects of heat stress on membrane phospholipid metabolism andβ-adrenergic receptor of hepatocyte from hypertherm1ia rat were investigated. The contents of phospholipids includin phosphatidylinositol(P1) phosphatidylsevine ( PS ), phosphatidylethanolamine(PE ) and phosphatidylcholine (PC)were determined by high performance liquid chromatography, the activity of phospholipase A2: (PLA2)by modified hydrochloric acid titration method, the content of arachidonic acid(AA)by gas-chro- matography,while the maximal binding capacity (Bmax)and Kd ofβadrenergic receptor(β-AR)were identified by radioligand binding assay。 The resuIts showed that heat stress increased the activity of PLA2, and the content of AA,but the contents of phospholipids such as PI,PE and PC decreased, However these aIterations were blocked by pretreatment of rat with mepacrine (20mg/kg ip,1 h before heat stress). No significant difference was found between the values of all phospholipids parameters tested and normal level. The production of AA and lysophosphOlipids which mediated tissue injury was attenuated。 On other hand,it was revealed in the experiment that the decrease of Bmax and the increase of affinity of the β-AR were induced by heat stress。 The pretreatment with mepacrine may recover the affinity ofβ- AR near to normal value,but the Bmax'of βAR keeps reducing because it was demonstrated priviOualy that mepacrine is a fullβ-AR agonist and could prevent tissues and organs from the heat injury。
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1995年第3期163-166,共4页
Medical Journal of Chinese People's Liberation Army
