摘要
为了探讨抗原特异性CD4+T细胞在体内的分裂、表型、Th1细胞因子的产生和组织器官的分布。将CFSE标记的抗原特异性初始CD4+T细胞静脉被动输给小鼠后,进行免疫,3d后处死小鼠取其脾脏、淋巴结和肺组织,分离单个核细胞,利用流式细胞计数仪在单个细胞水平上,观察细胞的分裂、表型、Th1细胞因子的产生和组织分布。结果显示在没有抗原刺激的情况下,未见初始CD4+T细胞分裂,其主要分布于淋巴结和脾脏。当受到抗原刺激后,CD4+T细胞分裂1～5次,主要分布于脾脏和肺组织,CD25的表达增加,CD62L的表达随着细胞分裂次数的增加而减少。IL-12促进CD25的表达和细胞的分裂。促进Th1细胞的分化和IFN-γ的表达。研究的结果提示,在体内,当CD4+T细胞活化后,主要分布于脾和非淋巴组织发挥其免疫效应。
The division, phenotypes, production of the Thl type cytokines and tissue distribution of the antigenspecific naive CD4^＋ T cells were to be investigated in the present study, in which the antigen-specific CD4^＋T cells were isolated from OVA-TCR transgenic mouse（TCR-Tg）, labeled with CFSE and adoptively transferred into normal BALB/c mice. After immunization with OVA, the mononuclear cells were prepared from spleens, lymph nodes and lungs, and the division, phenotypes, production of the Th1-type cytokines and tissue distribution were determined by FACS. It was demonstrated that the antigen-specific naive CD4^＋ T cells were primarily located in lymph nodes and spleens without any antigenic stimulation, whereas, after in vivo stimulation with antigen, the cells divided 1-5 times, and were located mainly in spleens and lungs. With the increasing in times of cell divisions the expressions of CD25 and CD62L molecules were enhanced or reduced respectively. IL-12 could enhance cell divisions, expression of CD25, differentiation of the Th1 cells and the expression of IFN-γ. It is evident that the antigen specific naive and effector CD4^＋T cells express different cell surface molecules and produce different cytokines.
出处
《现代免疫学》
CAS
CSCD
北大核心
2005年第4期279-283,共5页
Current Immunology
基金
国家自然科学基金创新群体项目(30321004)
广东省自然科学基金重点项目(04105349)
广州市科技局创新药物专项(2004Z3-E4031)。
作者简介
杨滨燕（1955-），女，黑龙江人，副主任技师，学士，主要从事细胞与分子免疫学研究。
吴长有（电话：020-87331552；E-mail：chang_you_wu@yahoo.com）
