摘要
目的比较观察抗原特异性初始CD4+T细胞和效应性/记忆性Th1细胞在体内外IFN-γ的产生和在淋巴组织的分布。方法将标记相同数量的OVA-抗原受体转基因小鼠(OVA-TCR-Tg)的初始CD4+T细胞和Th1细胞被动输给正常小鼠后,利用流式细胞仪在单个细胞水平上观察初始CD4+T细胞和Th1细胞在体内淋巴组织器官的分布和IFN-γ的产生。结果体外经抗原刺激后,大于98%的Th1细胞和4.1%的初始CD4+T细胞表达IFN-γ。当被动输入机体后,初始CD4+T细胞主要分布于淋巴结,而Th1细胞主要分布于脾脏。当再次受到抗原的刺激后,记忆性Th1细胞能迅速产生IFN-γ。初始CD4+T细胞与效应/记忆Th1细胞在淋巴结和脾脏不同的分布,可能与CD62L的表达有关。结论本研究在我们以前研究的基础上进一步探讨了CD4+T细胞和记忆性Th1细胞的差异。此结果为进一步揭示初始CD4+T细胞与Th1细胞的差异提供了实验和理论依据。
Objective To assess the IFN-γ production and distribution of naive CD4^+T cells as well as effector/memory Th1 cells in lymph nodes and spleen. Methods Naive CD4^+T cells from OVA-TCR-transgenic mice (OVA-TCR-Tg) were isolated and Th1 cells were generated in vitro in the presence of APC and OVA peptide antigen under Th1-polarized culture condition. Equal number of CFSE labeled naive CD4^+T cells and Th1 cells were adoptively transferred into normal mouse. The expression of IFN-γ and distribution of naive CD4^+T and effector/memory Th1 cells in lymphatic nodes and spleen were assessed by FACS. Results More than 98% Th1 cells and 4.1% naive CD4^+T cells had expressed IFN-γ following stimulation of antigen in vitro. After transferred into mice, the naive CD4^+T cells were preferentially distributed in lymphatic nodes, while the effector/memory T cells were localized mainly in spleen, in which cells could rapidly produce IFN-γ following re-exposure to the same antigen. The difference of naive CD4^+T and effector/memory Th1 cells in the distribution in lymphtic nodes and spleen was likely related to the high expression of CD62L on naive CD4^+T cells. Conclusion Naive CD4^+T cells and effector/memory Th1 cells reveal the change of surface CD62L expression, IFN-γ production, and distribution in lymphatic organs, which provides a useful information for understanding difference between naive and memory T cells in their biological function and tissue distribution.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2005年第4期273-276,共4页
Immunological Journal
基金
国家重点基础研究发展计划项目(2001CB510007)
广州市科技局创新药物专项(2004Z3-E4031)
国家自然科学基金创新群体项目(30321004)资助
