摘要
目的分离纯化麦冬多糖抗心肌缺血的活性部位,得到相对分子质量均一的组分 MDG-1,并进行结构分析。方法水提醇沉得麦冬粗多糖,超滤后经 DEAE Sepharose FF 柱和 Sephadex G25柱色谱纯化后得 MDG-1。经完全酸水解、高碘酸氧化、Smith 降解、甲基化分析、~1H NMR 及^(13)C NMR 等研究分析 MDG-1的化学结构。结果和结论MDG-1为β-D-果聚糖,易溶于水,数均分子量(M_n)为3400,重均分子量(M_w)为4800,峰尖分子量(M_p)为5000,以2→1连接的呋喃型果糖为主,平均每2.8个主链残基上有一个 Fruf(2→6)Fruf(2→分支)。该多糖还含有微量葡萄糖(果糖:葡萄糖=35:1),可能连接在多糖的还原端。
Aim To separate and purify the anti-myocardial ischemic polysaccharide fraction with a homogenous molecular weight from Ophiopogon japonicus,then study the chemical structure of the parts. Methods Crude polysaccharides were prepared by extracting the tube root fraction of Ophiopogon japonicus with water,then precipitation with ethanol.From the crude polysaccharides,the polysaccharide of MDG-1 was separated and purified using ultrafiltration,DEAE Sepharose FF and Sephadex G-25 column chromatography.Its structure was studied by complete hydrolysis,periodate oxidation,Smith degradation, methylation analysis,~1H NMR and ^(13)C NMR analysis etc.Results and Conclusion MDG-1 was a water- soluble β-D-fructosan,containing a backbone composed of Fruf(2→1),and a branch of Fruf(2→6)Fruf (2→per average 2.8 of main chain residues.M_n,M_w and M_p of MDG-1 were 3 400,4 800 and 5 000, respectively.MDG-1 contains trace of Glc,which maybe connect to its reducing terminal.Molar ratio of Fru and Glc is approximately 35:1.
出处
《药学学报》
CAS
CSCD
北大核心
2005年第7期636-639,共4页
Acta Pharmaceutica Sinica
基金
国家高技术研究发展计划(863计划)资助项目(2002AA2Z3224).
关键词
麦冬
多糖
结构
Ophiopogon japonicus
polysaccharide
structure
