摘要
果实所处的微域环境对果皮组织中5-核苷酸酶活性有很大影响。无论套袋与否,均以树冠西南方位果实的酶活性最高。由于套袋提高了微域环境温度,果实5-核苷酸酶活性均显著高于不套袋处理。不同类型果袋所形成的微域环境不同,对果实5-核苷酸酶活性影响的效应有异。在一定范围内,缓慢持续升温或起伏升温都有利于果实5-核苷酸酶活性的提高,但如果升温时间太短,或温差变化太大,则不利于酶活性的提高。果实在35℃、40℃或48℃处理温度下,高湿状态均有利于果实5-核苷酸酶活性的提高。4种外源活性氧处理均使果实5-核苷酸酶活性显著下降,而外源CaCl2处理果实酶活性比对照提高了55.39%。
The present experiment was conducted to reveal the effect of microenvironments and exogenous substance application on 5'-nucleotidase activities in apple peel tissue. By means of enclosing apple fruits in bags,treating with exogenous active oxygen species and regulative agents or placing them under controlled conditions at different fruit temperatures or relative humidity, the 5'-nucleotidase activities were compared with the corresponding controls. The results indicated that, a considerable effect of microenvironments was found on 5'-nucleotidase activities in fruit peel tissue. The highest enzymatic activity appeared in fruits on southwest canopy, regardless of bagged or non-bagged fruits, significantly higher than those from any other exposures. Fruits with bags had a significantly higher 5'-nucleotidase activity than the exposed ones. A variation of enzymatic activities was observed in fruits enclosed with different types of bags, which were supposed to alter the microenvironments around them. Within a certain range, gradual or fluctuant rise of fruit temperatures could favor the increase of 5'-nucleotidase activities as a result of heat adaptation,whereas the activity would be inhabited if the temperature-rising period was too short or temperature differential was too huge. No matter what temperatures fruits were subjected to, high relative humidity was favorable to stimulating the 5'-nucleotidase activities, which might partly explain why fruit sunburn wouldn't happen in humid climates. Treatments with four kinds of exogenous active oxygen species could reduce the 5'-nucleotidase activities significantly but spraying with CaCl_ 2 was able to enhance 5'-nucleotidase activities by 55.39%, reaching a significant level.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第2期191-196,共6页
Acta Horticulturae Sinica
基金
河北省自然科学基金资助项目(303203)
河北农业大学回国留学人员科研启动基金资助项目(20020915)
关键词
苹果
微域环境
外源物质
果实
5’-核苷酸酶
酶活性
Apple
Microenvironment
Exogenous substance
Fruit
5'-nucleotidase
Enzymatic activity
