摘要
为揭示低温锻炼下菊花生理变化特点及特异性表现,以切花菊(Chrysanthemum morifolium)秋白菊叶片为试验试材,在4℃下对其进行5 h的低温锻炼,然后在-5℃下分别进行0,0.5,1,3和5 h不同时间的低温胁迫。通过对其相对电导率、丙二醛(MDA)和脯氨酸含量及5′-核苷酸酶、过氧化物歧化酶(POD)和超氧化物歧化酶(SOD)活性的测定,研究了低温锻炼对菊花抗寒性的诱导。结果表明,低温锻炼的菊花叶片相对电导率及MDA含量比对照减小,而脯氨酸含量、SOD和5′-核苷酸酶活性比对照增加,说明低温锻炼在一定程度上提高了菊花植株的抗寒性。
To investigate the effects of cold acclimation on the cold tolerance, the leaves of chrysanthemum ( Chrysanthemum morifolium) were treated at 4 ℃ for 5 hours, and then the changes of electrolytic leakage, content of malondialdehyde level (MDA), soluble protein, proline and activity of 5'-nucleotidase, superoxide dismutase (SOD)and peroxidase (POD)were measured after low temperature stress with different times (0.5- 5 h).The results showed that the electrolytic leakage and the content of MDA in leaves treated with cold acclimation were lower than those of non-treated, the contents of proline and the activities of SOD and 5'-nucleotidase kept higher levels compared with those of controls. The results indicated that the cold tolerance of chrysanthemum was improved by cold acclimation treatment.
出处
《华北农学报》
CSCD
北大核心
2009年第4期179-182,共4页
Acta Agriculturae Boreali-Sinica
基金
河北农业大学留学回国人员科研启动基金项目(2004-897)
教育部留学回国人员科研启动基金项目(2006-1018)
关键词
菊花
低温锻炼
低温胁迫
抗寒性
Chrysanthemum morifolium
Cold acclimation
Low temperature stress
Cold tolerance
作者简介
李云(1981-),女,河北安国人,硕士,主要从事花卉抗性生理和育种研究。
通讯作者:杨际双(1970-),男,山东郓城人,副教授,博士,主要从事花卉栽培生理与育种教学和科研工作。
通讯作者:张钢(1959-),男,河北栾城人,教授,博士,主要从事观赏植物抗性生理生态研究。