摘要
目的:探讨中药三氧化二砷(As2O3)对人胃癌多药耐药细胞系(SGC7901/ADM)的耐药逆转及凋亡诱导作用。方法:应用免疫细胞化学方法(PV法)测定不同剂量的As2O3用药前后细胞内mdr-1基因产物P-gp的表达变化;同时用流式细胞仪对以上各组细胞进行细胞周期解析和凋亡率的测定。结果:As2O3在0.10～0.75μmol/L浓度范围内能够逆转SGC7901/ADM细胞内P-gp的表达,并将细胞阻滞于G0～G1期、诱导细胞凋亡(P<0.01),其作用随As2O3剂量增加而增大。结论:在胃癌耐药细胞系SGC7901/ADM中,As2O3具有耐药逆转和凋亡诱导双重作用,并呈剂量依赖性。
Objective: To investigate MDR reversing and apoptosis inducing effects treated with arsenic trioxide (As2O3) in gastric cancer cell line SGC7901/ADM, which appears multidrug resistance. Methods: Immunocytochemistry (PV-method) was used to detect the different expression level of P-glycoprotein (P-gp,mdr-1 gene product), and flow cytometry (FCM) was used to check cell cycle, treated with or without different concentrations of As2O3 in SGC-7901/ADM cell line. Results: Reversing effect of P-gp expression was found in SGC7901/ADM cell line when treated with different concentrations of As2O3, ranging from 0.1μmol/L to 0.75μmol/L, and cell cycle was blocked at the period of Go-G1, augmented with the increasing concentration of As2O3 (P<0.01). Conclusions: Arsenic trioxide may play a role in the regulation of MDR reversing and apoptosis induced by the way of dose-dependent in gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2005年第11期611-613,共3页
Chinese Journal of Clinical Oncology
基金
国家中医药管理局科研基金资助(编号:00-01LL13)
