大鼠视网膜缺血再灌注后多聚二磷酸腺苷核糖聚合酶的表达变化被引量：2

EXPRESSION OF PALY ADP-RIBOSE POLYMERASE AFTER RETINAL ISCHEMIA REPERFUSION IN RATS

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摘要目的观察大鼠视网膜缺血再灌注损伤后不同时相视网膜结构变化及多聚二磷酸腺苷核糖聚合酶(PARP)的表达情况。方法升高眼压到110mmHg持续1h,建立大鼠视网膜缺血再灌注模型。HE染色观察视网膜组织病理学改变;免疫组化ElivisionTM法检测视网膜细胞PARP表达。结果正常对照组大鼠视网膜仅见微量PARP表达;缺血1h再灌0h组视网膜结构未见明显变化;再灌注6h,视网膜开始有中性粒细胞浸润,神经节细胞层(ganglioncelllayer,GCL)PARP表达显著增加;12、24h组视网膜中有较多中性粒细胞浸润,神经节细胞层PARP表达24h达高峰后下降,但仍维持较高水平;24h后内核层(innernuclearlayer,INL)细胞PARP表达明显增高,168h达高峰,伴之神经节细胞层和内核层细胞明显变薄。另外,也见自身对照眼视网膜神经节细胞层PARP表达增加。结论大鼠视网膜缺血再灌注早期PARP即表达上调,24h组在GCL达高峰,168h组在INL达高峰,这一过程可能与视网膜神经节细胞层和内核层细胞凋亡有关。 Objective To investigate the expression of Poly ADP-Ribose Polymerase(PARP) and morphological changes after varies duration of retinal ischemia reperfusion in rats. Methods The retinal ischemia reperfusion model was developed by increasing intraocular pressure to 14.63kPa in rat eyes. Morphological changes of the rat eyes were observed by means of routine histopathology with HE staining. Immunochemical techniques were used to evaluate PARP positive expression in retina after varies duration of reperfusion. Results The minute expression of PARP was found in normal control group and o hour after reperfusion group, of which histopathological changes were not revealed in the rat retinas. Retinal ganglion cell layer (RGL) and inner nuclear layer(INL)of the retina were observed, however, which became significantly thinner 1hour after ischemia and then reperfused from 24 to 336 hours. Furthermore, PARP expression began to increase after retinal ischemia for 1hour in RGL, peaked at 24 hours after reperfusion, and decreased thereafter, continued at high level at 72 hours after reperfusion, then another peak was demonstrated that PARP positive cells were localized in inner nuclear layer (INL) of the retina at 168 hours after reperfusion. A few neutrophils infiltrated in the retina at 6 hours after reperfusion. As time elapsed,the number of infiltrated neutrophils increased, and lots of neutrophils were observed at 12 and 24 hours after reperfusion. Conclusion PARP expression in retina are observed at 6 hours and peak at 24 hours after reperfusion in RGL. Another peak demonstrate that PARP positive cells are localized in INL of the retina at 168 hours after reperfusion. Ischemia reperfusion insult of the eye may remarkably damage the retina of the rat eye. The damage to retinal cells is mainly localized within RGL and INL. Apoptotic cell death may be involved in the pathogenesis of retinal disorder.

作者郭闯张宇新刘学均张作凤周宏霞

机构地区华北煤炭医学院人体解剖学教研室

出处《中国煤炭工业医学杂志》 2005年第5期526-528,共3页 Chinese Journal of Coal Industry Medicine

关键词视网膜缺血再灌注多聚二磷酸腺苷核糖聚合酶细胞凋亡大鼠 retina ischemia reperfusion Poly ADP-Ribose Polymerase apoptosis rats

分类号 R-332 [医药卫生]

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