摘要
背景与目的:近年来关于nm23、上皮型钙粘素(E-cadherin)和连环素(Catenins)与肿瘤关系的研究比较多,但不同研究得到的结果差异较大。而且近年来的研究显示,nm23、E-cadherin和Catenins还参与细胞信号转导,使它们在肿瘤转移过程中的作用可能具有多面性。本实验检测nm23、E-cadherin和β-Catenin在一组非小细胞肺癌(non-sm allcelllang cancer,NSCLC)中的表达水平,试图了解它们与非小细胞肺癌转移和预后的关系,以及它们之间的相互关系。方法:采用免疫组化(SP法)检测112例NSCLC和30例肺良性病变组织中nm23、E-cadherin和β-Catenin的表达水平。结果:(1)nm23、E-cadherin和β-Catenin在肺癌组织中表达水平(分别为53.0%,53.1%和47.2%)显著低于癌旁肺组织中的表达水平(分别为64.8%,79.7%和80.6%)和肺良性病变组织中的表达水平(分别为76.9%,83.5%和85.6%);(2)nm23、E-cadherin和β-Catenin在有淋巴结转移的肺癌组织中的表达水平(分别为48.0%,47.3%和41.2%)显著低于无淋巴结转移者(分别为65.0%,60.5%和60.3%),在Ⅲ期和Ⅳ期NSCLC的表达水平(分别为44.8%,46.6%和38.1%)均显著低于Ⅰ期、Ⅱ期NSCLC(分别为67.2%,64.3%和63.1%);(3)nm23、E-cadherin和β-Catenin高表达组5年生存率(分别为39.29%,35.85%和37.29%)
BACKGROUND & OBJECTIVE: Recently, studies showed nm23, E-cadherin, and Catenins play important roles in cellular signal transduction, which enhance complexity of their functions in tumor metastasis and can partly explain the diversity of the results from different studies. This study was to investigate correlations of expressions of nm23, E-cadherin, and β-Catenin in non-small cell lung cancer (NSCLC) to metastasis and prognosis, and their interrelations. METHODS: Expressions of nm23, E-cadherin, and β-Catenin in 112 specimens of NSCLC and 30 specimens of benign pulmonary lesion were detected by SP immunohistochemistry. RESULTS: The expressions of nm23, E-cadherin and β-Catenin were significantly weaker in NSCLC tissues than in adjacent non-cancerous tissues, and benign pulmonary tissues (53.0% vs. 64.8%, and 76.9%, P < 0.01; 53.1% vs. 79.7%, and 83.5%, P < 0.01; and 47.2% vs. 80.6%, and 85.6%, P < 0.01), significantly weaker in NSCLC tissues with lymph node metastasis than in those without metastasis (48.0% vs. 65.0%, P < 0.01; 47.3% vs. 60.5%, P < 0.01; and 41.8% vs. 60.3%, P < 0.01), and significantly weaker in NSCLC tissues of stage Ⅲ-Ⅳ than in those of stage Ⅰ-Ⅱ (44.8% vs. 67.2%, P < 0.01; 46.6% vs. 64.3%, P < 0.01; 38.1% vs. 63.1%, P < 0.01). The 5-year survival rates of patients with weak expressions of nm23, E-cadherin, and β-Catenin were significantly lower than those of patients with strong expressions (3.6% vs. 39.3%, P < 0.01; 6.8% vs. 35.8%, P < 0.01; and 3.8% vs. 37.3%, P < 0.01). CONCLUSIONS: Down-regulations of nm23, E-cadherin, and β-catenin closely relate to metastasis of NSCLC. Detection of nm23, E-cadherin, and β-Catenin might be helpful to predict prognosis of NSCLC patients.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2005年第5期616-621,共6页
Chinese Journal of Cancer
基金
国家自然科学基金项目(No.30070333)~~
关键词
肺肿瘤
NM23
钙粘素
连环素
免疫组化
Lung neoplasms
nm23
E-cadherin
β-Catenin
Immunohistochemistry
