摘要
优化了粳稻的组织培养体系;通过Gus基因的瞬间表达研究,明确了以DNA金弹到靶细胞距离为9cm,轰击2次时,PDS1000/He基因枪法转化水稻胚性愈伤组织的效率最高。应用基因枪法将抗除草剂基因(bar)转入吉林省主栽的水稻品种长白8号中,获得了可育的转基因水稻植株。经过Gus基因的组织化学分析,PCR和Southern杂交检测,证明抗除草剂基因已整合到水稻基因组中得以表达,使水稻产生对除草剂的抗性。转基因水稻中发现一例基因沉默现象。
A herbicide gene(bar) was delivered into a commercial rice variety 'Changbai 8' cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun(PDS-1000/He) transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants (D0-1, D0-2 and D0-3) showed herbicide resistance. Gene silence was observed in one transgenic rice plant (D0-5).
出处
《吉林农业科学》
CSCD
2005年第2期3-6,共4页
Journal of Jilin Agricultural Sciences
