摘要
文中对抗除草剂转基因玉米的研究进行了综述。抗除草剂转基因玉米的转化方法主要有基因枪法和农杆菌介导法,其中基因枪法是转基因技术中经常使用的方法;也是在玉米中应用最成功的方法;农杆菌介导法因其具有费用低、拷贝数少、基因沉默现象少、能转化较大片段、遗传稳定性较高、再生植株可育性较好等优点也越来越受到重视。通常以Bar基因作为标记基因,采用喷施除草剂和PCR检测相结合的方法对抗除草剂转基因玉米进行检测和筛选。抗除草剂转基因玉米的安全性问题主要涉及演化为田间杂草的可能、基因漂移和田间杂草的抗药性3个方面。
The studies on the herbicide-resistant GM maize were reviewed, and the herbicide-resistant GM maize transtormation methods mmnly include particle bombardment and agrobacterium-mediated transformation, the particle bombardment is the commonly used and the most success- ful transgenic technique; while the agrobacterium-mediated transformation, due to its low costs, fewer copies, less gene silencing, can be trans- formed into larger fragments with higher genetic stability and higher fertility of regenerated plants, etc. With the Bar gene as a marker gene, the herbicide-resistant GM maize was determined and screened with herbicide spraying of herbicides and PCR. The shfety issues of herbicide-resistant GM maize mainly involve the possible evolution of weed, weed gene flow and resistance to herbicide.
出处
《安徽农业科学》
CAS
2012年第14期8012-8013,8017,共3页
Journal of Anhui Agricultural Sciences
关键词
玉米
抗除草剂
安全性
Maize
Herbicide-resistant
Security
作者简介
作者简介魏俊杰（1974-），女，河北保定人，讲师，硕士，从事遗传与育种研究，E-mail：junjiewei@126．com
