摘要
使用Ｐｒｏα１（Ⅰ）、Ｐｒｏα１（Ⅲ）人胶原ｃＤＮＡ探针，采用ｃＤＮＡ－ｍＲＮＡ班点杂交技术，观察了染尘２个月、４个月的矽肺大鼠及汉防己甲素治疗１个月和３个月的矽肺大鼠肺组织中Ⅰ型、Ⅲ型胶原的ｍＲＮＡ水平的改变情况。实验结果表明，矽肺组织中Ⅰ、Ⅲ型胶原ｍＲＮＡ的含量比正常肺组织明显增加（Ｐ＜０．０５），汉防己甲素治疗后Ⅰ、Ⅲ型胶原正常含量则较矽肺组织显著减少。因此，我们认为矽肺病变组织中胶原纤维的积聚是由石英粉尘引起胶原基因表达改变所致；汉防己甲素能直接或间接地抑制胶原基因的转录，从而抑制矽肺病变中胶原蛋白的合成。
Human Proα1(Ⅰ)、Proα1(Ⅲ) 3 cDNA probes and RNA dot blot method were employed to determine the levels of collagen mRNA in the lung of silicotic rats treated by TT. The results showed that Proα1(Ⅰ) and Proα1(Ⅲ) mRNA levels increased significantly at 60 and 120 days after the rats were exposed to silica dust. The mRNA levels went down at 1 and 3 months after treated by TT. This result suggests that TT may inhibit the gene expression of lung collasen in silicosis.
出处
《卫生研究》
CAS
CSCD
北大核心
1994年第4期201-203,共3页
Journal of Hygiene Research
关键词
矽肺
胶原基因
汉防己甲素
Silicosis
collagen gene
tetrandrine
mRNA
dot blot
