摘要
[目的]观察补肾方含药血清对白细胞介素1(IL-1)抑制软骨细胞增殖及诱导软骨细胞产生一氧化氮(NO)作用的 影响。[方法]新西兰兔4只,每只均灌服1.15 kg/L的补肾方2.5 mL,连续3 d,制备含药血清;取体外培养的兔软骨细 胞,分别加入含1、10、20 μg/LIL-1的培养液,确定IL-1对软骨细胞增殖的抑制浓度;补骨方组分别加入20μg/L的IL-1和 不同浓度的补肾方含药血清,模型组加入20 μg／L的IL-1及不同浓度的空白血清,培养3 d后用四甲基偶氮唑盐(MTT)法 检测软骨细胞的增殖,Griess法检测NO的产生。[结果]选择20μg/L浓度的IL-1抑制软骨细胞的增殖;不同浓度的补肾方 含药血清均能拮抗IL-1抑制软骨细胞增殖的作用,抑制IL-1诱导软骨细胞产生NO。[结论]补肾方能直接拮抗或通过NO 介导间接拮抗IL-1抑制软骨细胞增殖的作用,这可能是补肾方治疗骨性关节炎(OA)的机制之一。
[Objective] To observe the effect of serum containing Bushen prescription (BP) on inhibition of chondrocyte proliferation and nitric oxide (NO) production in chondrocyte induced by interleukin-1 (IL-1). [Methods] Four New Zealand rabbits were administered BP 2.5mL (1.15kg/L) for 3 days for the preparation of BP-containing serum. Rabbit chondrocytes were cultured in-vitro and were added with DMEM culture containing IL-1 1, 10 and 20 μg/L to examined the inhibition concentration of IL-1 for chondrocyte proliferation. After then, BP group was added with 20 μg/L IL-1 and various concentrations of BP-containing serum and the model group with 20 μg/L IL-1 and various concentrations of blank serum. Alter 3-day culture, chondrocyte proliferation were dectected by thiazolyl blue colorimetry (MTT) and NO content by Griess method. [Results] The inhibition concentration of IL-1 for chondrocyte proliferation was 20 μg/L; various concentrations of BP-containing serum could counteract chondrocyte proliferation and inhibit NO pnxluction in chondrocyte induced by IL-1. [Conclusion] BP can counteract chondrocyte proliferation directly or indirectly by the mediation of NO, which may be one of its therapeutic mechanisms in treating osteoarthritis.
出处
《广州中医药大学学报》
CAS
2005年第2期138-140,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药管理局资助科研课题(编号:103020)
