摘要
目的观察IP_6对体外培养的肝癌7402细胞凋亡的影响。方法用透射电镜、琼脂糖凝胶电泳和流式细胞仪观察IP_6处理细胞后其生化和形态学指标的改变。结果透射电镜观察7402细胞经IP_6处理6h后,细胞核固缩,形成着边现象,至24h部分细胞脱壁,琼脂糖凝胶电泳呈现梯形条带,流式细胞仪分析显示处理细胞中出现亚二倍体峰,证实处理组细胞发生凋亡。结论 IP_6能诱导肝癌7402细胞凋亡,或许诱导细胞凋亡是IP_6的抗癌机理之一。
Objective Observing the effects of IP6 on the apoptosis in human hepatoma cell line, 7402, Methods The apoptsis was detected by trasmissional electron microscope, gel electrophoresis and flowcytometry. Results After 6h of treatment with IP6, we can observed the typical nuclear condensation under transmissional electron microscope. After 24h of treatment, most of cells detached, characteristic DNA ladder and decreased DNA content was showed. Conclusion Apoptosis of 7402 cell can be induced by IP6. The anticancer function of IP6 is by apoptosis partially.
出处
《现代消化及介入诊疗》
1999年第2期37-39,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
